摘要
目的探讨白细胞介素13(IL-13)对肾小球系膜细胞分泌白细胞介素12(IL-12)的调节作用。方法用酶联免疫吸附(ELISA)法和逆转录聚合酶链反应(RT-PCR)法检测系膜细胞的IL-12蛋白水平和IL-12p40 mRNA的表达。结果未受刺激的系膜细胞无IL-12 mRNA的表达及其蛋白分泌。LPS诱导系膜细胞的IL-12p40 mRNA的表达及其蛋白分泌。IL-13在1～100ng/ml的浓度范围内呈剂量依赖性抑制LPS诱导的系膜细胞的IL-12分泌及其 mRNA的表达。结论LPS诱导系膜细胞分泌IL-12,而IL-13则抑制LPS诱导的系膜细胞IL-12的表达;IL-13可能通过抑制IL-12的产生,调整了体内Th1/Th2细胞因子的平衡,作为抗炎性细胞因子在肾小球肾炎发病机制中发挥一定的作用。
[Objective] To investigate the effect of interleukin-13（IL-13） on interleukin-12（IL-12） produced in mesangial cells. [Methods] The protein synthesis of IL-12 in mesangial cells was measured by ELISA. The expression of IL-12 mRNA in mesangial cells was evaluated by RT-PCR. [Results] The production of IL-12 in mesangial cells stimulated by LPS was significantly increased（P 〈0.01）. IL-13（1～100 ng/mL） inhibited the protein and mRNA expression of IL-12 in a dose-dependent manner （P 〈0.05 or P 〈0.01）. [Conclusion] IL-13 could inhibit IL-12 expression induced by LPS in mesangial cells. IL-13 may regulate renal injury and immune responses by balancing Th1/Th2 in glomendonephritis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第13期1541-1544,共4页
China Journal of Modern Medicine
