摘要
目的观察幽门螺杆菌(H.pylori)培养滤液作用于胃癌细胞后,细胞色素氧化酶Ⅱ(COX-Ⅱ)mRNA及其蛋白表达的变化,探讨H.pylori致胃癌的可能分子机制。方法将6μl/mlH.pylori培养滤液与胃癌细胞分别作用4、8、12小时,收集细胞,提取RNA,应用RT-PCR和Western blot法检测各时间点COX-ⅡmRNA及其蛋白表达。结果正常情况下,胃癌细胞COX-ⅡmRNA稳定表达。H.pylori培养滤液分别作用4、8、12小时后,其表达量呈缓慢下降趋势,8、12小时的表达量显著低于对照组(P<0.05)。COX-Ⅱ蛋白的表达呈现相似的趋势。结论H.pylori可引起胃癌细胞线粒体编码基因COX-ⅡmRNA和蛋白的表达障碍,影响线粒体功能。
Objective To study the effects of concentrated H. pylori culture supematant（CHCS） on expression of COX-Ⅱ mRNA and protein encoded by mtDNA in gastric carcinoma cells, and explore the potential role of H. pylori in the gastric carcinogenesis. Methods After treated with 6μl/ml CHCS for 4, 8.12h,gastric carcinoma cells were collected and the RNA and protein from the cells at different times were extracted. Expression of COX-Ⅱ mRNA and protein was detected by RT-PCR and Western blot. Re- suits Expression of gastric cancer cells COX-Ⅱ mRNA behaved steadily. After H. pylori culturing filtrate incubated for 4h,8h, 12h respectively, the expression decreased slowly. Especially the expression for 8h, 12h was dramatically lower than that of control group（ P 〈 0.05 ）. Expression of COX-Ⅱ protein revealed the same trend. Conclusions H. pylori could affect mitochondrial function by down-regulating the expression of mitochondrial encoding cvtochrome oxidase Ⅱ gene.
出处
《临床内科杂志》
CAS
2007年第7期493-495,共3页
Journal of Clinical Internal Medicine
基金
国家自然科学基金(39870345)
军内"十五"重点课题(01Z075)资助
关键词
幽门螺杆菌
胃癌细胞
线粒体
细胞色素氧化酶Ⅱ
Helicobacter pylori
Gastric carcinoma cell
Mitochondria
Cytochrome oxidase subunit ]I
