摘要
目的：检测慢性粒细胞白血病ｂｃｒ基因重排。方法：应用荧光原位杂交技术，以自行筛选和制备的酵母人工染色体为探针进行荧光原位杂交，检测慢性粒细胞白血病ｂｃｒ基因的重排。结果：用２２号染色体ｂｃｒ基因附近的ＹＡＣ７６５Ｅ３，在９例慢性粒细胞白血病中，发现５例慢性期患者，２例急变期患者和１例经α干扰素治疗后的患者存在ｂｃｒ基因重排，１例自体骨髓移植后患者的核型呈嵌合状态，即同时存在正常的和具有ｂｃｒ基因重排的核型。结论：ＹＡＣ７６５Ｅ３是一个有用的检测ｂｃｒ基因重排的探针，荧光原位杂交技术可能成为白血病治疗效果的监测和微量残留病检出的一种重要手段。
Objective:To detect bcr gene rearrangement in chronic myeloid leukemia(CML).Methods:Bcr rearrangement was detected by fluorescence in situ hybridization (FISH),using 765E3,a yeast artificial chromosome (YAC) derived probe flanking the breakpoint within bcr gene.Results:Nine patients with CML were examined, bcr gene rearrangement was revealed in 5 cases in chronic phase,2 in blastic phase,and one after interferon α therapy.The karyotype of 1 case after autologous bone marrow transplantation (ABMT) was chimera with normal and bcr gene rearrangement chromosomes.Conclusion:YAC765E3 is a useful probe for detecting bcr gene rearrangement.FISH technique is likely an important tool for monitoring of treatment and revealing minimal residual disease in CML.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1997年第6期308-310,共3页
Chinese Journal of Hematology
基金
卫生部科学研究基金
中国博士后科学基金
关键词
基因
BCR
荧光原位杂交
慢性
白血病
基因重排
Leukemia,myeloid, chronic Gene,bcr Fluorescence in situ hybridization Yeast artificial chromosome
