摘要
采用离体接种法研究了不同苹果品种被轮纹病菌(Physalospora piricola Nose)侵染后苯丙氨酸解氨酶(PAL)活性以及木质素和绿原酸含量的变化及其与抗病性的关系。接种6d 后各品种(系)枝条的 PAL 活性迅速提高,两天内提高几百倍,果实的 PAL 活性也提高很多;伴随PAL 酶活性的变化,各品种果实、枝条的绿原酸、木质素含量也发生明显的变化,但抗病品种(系)与感病品种差异并不显著。结果表明苹果对轮纹病菌的抗性与苯丙酸类代谢的定速酶 PAL 有关,并通过其代谢产物木质索、绿原酸得到表现,但 PAL 酶活性并不宜作为苹果抗轮纹病生理指标。
Fruits and twigs of four apple varieties with different resistance to ring rot of apple were inoculated with Physalospora piricola Nose.Six days after inoculation all varieties appeared to increase rapidly in enzymatic activity of phenylalanine ammonialyase(PAL)in twigs.Within two days,it was increased by a few hundred times.The activity of PAL in fruits also increased much.With the increase of PAL activity,the content of chlorogenic acid and lignin in fruits and twigs changed obviously.However,the difference between resistant and susceptible varieties was not significant.The results showed that there was relationship between the ac- tivity of PAL as well as the amount of chlorogenic acid and lignin and the resistance of apple to ring rot,yet there was not a positive or negative correlation between them.
出处
《果树科学》
CSCD
北大核心
1997年第3期149-152,共4页
Journal of Fruit Science
关键词
苹果
抗病机理
苯丙酸代谢
轮纹病
Apple
Resistance
Metabolism of phenylalanine
Physalospora piricola Nose
