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玻璃化冷冻保存小鼠生发泡期卵母细胞的研究 被引量：1

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摘要 试验探讨了卵母细胞形态、基础液等因素对小鼠生发泡期卵母细胞冷冻效果的影响,目的在于筛选适合超低温冷冻保存小鼠卵母细胞的冷冻程序。通过这一途径可望建立卵母细胞种子库,并为体外受精、卵核移植和体细胞的克隆研究与应用提供试验材料。试验结果:①COC组、DO组卵裂率分别是23.17/、30.96/,差异不显著(P>0.05)。两者都与对照组(60.00/)差异显著(P<0.01)。②用DMEM(31.25/)和DPBS液(30.28/)作为玻璃化冷冻液的基础液效果差异不显著(P>0.05)。
作者 郭希萍 刘长松 崔成
机构地区 四川农业大学临床兽医系
出处 《畜牧与饲料科学》 2007年第3期23-25,共3页 Animal Husbandry and Feed Science
关键词 小鼠 GV期卵母细胞 玻璃化冷冻
分类号 Q954.4 [生物学—动物学]
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参考文献7

  • 1[1]Chen S U,Lien Y R,Chao K H,et al.Effects of cryopreservation on meiotic spindles of oocytes and it's dynamics after thawing:clinical implications in oocyte freezinga review article[J].Mol Cell Endocrinol,2003,202:101.
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  • 7李青旺,于永生,江中良,王立强,胡建宏,常万存.玻璃化冷冻保存牛卵母细胞技术研究[J].西北农林科技大学学报（自然科学版）,2003,31(1):109-112. 被引量：6

二级参考文献10

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畜牧与饲料科学
2007年 第3期

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