摘要
目的:分析人14-3-3蛋白家族的同源性及分子进化关系。方法:利用已公布的人基因组数据库,采用BLASTN程序检索人14-3-3蛋白家族各成员的编码基因和假基因,并利用DNAMAN软件进行序列联配,绘制其分子进化树。结果:该家族半数成员具有多个假基因序列,为返转座类型假基因。进化分析表明该家族有共同的祖先,可归为3个亚类。结论:人14-3-3蛋白家族每个成员长期进化所形成的多样性提示其功能具有独特性。
Objective： To determine the genomic structure of 14-3-3 protein family of Homo sapiens, and to analyze its molecular evolution. Methods： Based on the published genome database of 14-3-3 protein family of H.sapiens, the coding genes and pseudogenes of all 14-3-3 members were retrieved by using BLASTN software, and their genomic structures were analyzed. All the sequences were multialigned with DNAMAN software and the molecular evolution tree was drawn. Results： Most of all 14-3-3 members have pseudogenes which were the retrotransposons of coding genes. The evolution of this gene family showed that they had the common ancestor and clustered as three subgroup. Conclusion： As a result of long time evolution, the diversity of this family suggested that each member might have its specific functions.
出处
《生物技术通讯》
CAS
2007年第4期587-589,共3页
Letters in Biotechnology
基金
河南省自然科学基金项目(0511042300)
