摘要
目的观察甘草水提液对吸烟小鼠肺组织超氧化物歧化酶(SOD)和丙二醛(MDA)的影响。方法昆明种小鼠,随机分成3组,即正常对照组、吸烟模型组、甘草水提液组。正常对照组小鼠不吸烟,其余两组连续吸烟60 d,4次/d,30min/次,甘草水提液组2次/d给予蒸馏水灌胃对照,60 d后处死动物,取左上肺固定,石蜡包埋,切片HE染色观察,其余肺组织做成匀浆,检测肺组织匀浆中SOD活性,MDA含量。结果吸烟组小鼠肺内出现程序不等的慢性支气管炎及肺气肿,同时肺部SOD活性明显下降,MDA含量明显增加。吸烟后加服甘草水提液组小鼠肺部病理表现明显减轻,肺部的SOD活性增加,MDA含量下降。结论甘草水提液对小鼠长期吸烟所造成的支气管肺组织病理损害有保护作用,其作用机制可能与甘草在肺部发挥的抗氧化作用等有关。
Objective To investigate the protective effects of glycyrrhizae water - extraction on smoking - induced pulmonary injury in mice. Methods Kuming mice were randomly divided into 3 groups： healthy control group, model group and glycyrrhizae group. Except healthy control group, the others were exposed to cigarette smoke, qid, 30 mlnute/day for 60 days. At the same time, the mouse of glycyrrhizae group were given glycyrrhizae water - extracion, bid for 60 days, the other mouse were given distilled water,bid for 60 days. The pathologic changes of airways and lung tissues were determined. The level of MDA and the activity of SOD were measured. Results There were more chronic bronchites and emphysema in the model group than in the healthy control group. Cigarette smoking reduced the activity of SOD and increased MDA level. Compared with the model group, the pathologic injury of airways and lung tissues were reduced, the activity of SOD increased and the level of MDA decreased in glycyrrhizae group. Conclusion Glyrrhizae water - extract has the protective effect on smoking - induced injury. The mechanism may due to its antioxidation.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第7期1665-1666,共2页
Lishizhen Medicine and Materia Medica Research
关键词
甘草水提液
吸烟
肺组织
超氧化物歧化酶
丙二醛
Glycyrrhizae water - extraction
Smoking
Pulmonary injury
Superoxide dismutase
Malodialdenhyde
