摘要
目的探讨大鼠慢性支气管炎形成过程的不同阶段,气道上皮细胞间黏附分子ICAM-1的表达情况。方法混合烟雾吸入法建立慢性支气管炎形成的系列发展模型;SP法测定气道上皮细胞细胞间黏附分子ICAM-1的表达,并与病理形态学改变的半定量评分、支气管肺泡灌洗液中炎症细胞数及分类以及肺组织的髓过氧化物酶(MPO)活性进行相关性分析。结果在慢性支气管炎形成过程中,气道上皮ICAM-1的表达呈逐渐上调趋势,与气道病理变化及肺组织MPO活性呈正相关(分别为P<0.01,P<0.05)。结论大鼠慢性支气管炎形成过程中,ICAM-1为介导炎症的重要因子。
Objective To study the ICAM-1 expression on bronchial epithelial cells of the sequential smoke-induced chronic bronchitis rat model during the inflammation process. Methods Chronic bronchitis sequential rat model was established by passively inhaling smoke mixture. Experiments were performed on 30 young male Sprague-Dawley rats, which comprised 5 groups in random, i.e. 4 chronic bronchitis model groups and 1 control group. The levels of ICAM-1 expression were determined by immunohistochemistry method. Specimens were studied by semi-quantitative method to evaluate the morphologic changes in various stages. Meanwhile , the inflammatory cells of the BALF and the activity of MPO were analyzed. Results During the process of the chronic bronchitis, the ICAM-1 expression was increasing as time went on, positively related with the pathologic score（P 〈 0.01）, and the activity of MPO（P 〈 0.05）. Conclusion ICAM-1 may play an important role in the inflammatory process of chronic bronchitis.
出处
《中国微循环》
北大核心
2005年第5期305-307,T0001,共4页
Journal of Chinese Microcirculation
基金
上海市科学技术发展基金资助项目(004119060)
