摘要
研究一氧化氮（NO）合成酶抑制剂对孕鼠及胎鼠胎盘的影响，探讨NO在妊娠高血压综合征（妊高征）发病机制中的作用。选择清洁级Wistar大鼠，随机分为3组，自妊娠第14d开始皮下注射生理盐水、NO合成酶抑制剂亚硝基左旋精氨酸甲酯（L－NAME）每天125mg／kg或L－NAME每天250mg／kg，直到分娩。测量孕鼠尾动脉收缩压及胎鼠胎盘重量，测定孕鼠尿蛋白浓度及相关血液指标。结果：L－NAME使孕鼠产生剂量依赖性高血压和血细胞比容增加，并伴尿蛋白浓度及血尿素氮、肌酐、尿酸增加，血小板数减少。同时，L－NAME使胎鼠胎盘重量明显减轻、死胎增加，并见胎鼠后肢畸形，但不影响分娩时间。结论：NO合成酶抑制剂可使孕鼠产生妊高征的类似表现，表明妊娠期NO合成减少可能是妊高症发病机制中的一个重要因素。
PURPOSE Pregnancy-induced hypertension is associated with hypertension, proteinurea, fetal growth retardation and lesion of important organs. Our purpose was to determine the role of alteration in nitric oxide synthesis in the pathogenesis of pregnancy-induced hypertension.METHODS Saline solution, two different doses of L-nitro-arginine methyl easter (L-NAME) were in-fused subcutaneously to rats from day 14 of gestation. Systolic blood pressure, weight and mortality of pups,weight of placentas and day of spontaneous delivery were recorded. Maternal blood chemistry studies and urine protein were determined.RESULTS L-NAME caused a significant dose-dependent increase in systolic blood pressure and hematocrit. Maternal blood BUN, CRE, UA and urine protein were increased signiflcantly while blood platelet count decreased significantly compared with controls. This treatment also caused a substantial decrease in the weight of pups and placentas, with an increase in mortality, without affecting the gestational length. Besides, fetal hind-limb defects occurred with treatment with L-NAME. These signs did not show dose-dependence.CONCLUSIONS Infusion of an inhibitor of nitric oxide synthesis during pregnancy causes hypertension,proteinurea, lesion of kidney and fetal growth retardation, without affecting gestational length in pregnant rats.These signs are similar to those of pregnancy-induced hypertension, and indicate that a decrease in nitric oxide synthesis may be one of the important factors in the pathogenesis of this disorder.
出处
《上海医科大学学报》
CSCD
1997年第2期122-125,共4页
Journal of Fudan University(Medical Science)
关键词
一氧化氮合成酶
抑制剂
妊娠高血压
综合征
rat
inhibitor of nitricoxide synthase
fetal growth retardation
pregnancy-induced hypertension
