摘要
目的研究不同实验室对包装食品中蛋白质检测的实验误差。方法根据2000年全国包装食品消费的调查,选择了7类（谷物、豆类、肉类、奶制品类、饮料类、坚果、休闲食品）14种包装食品,在全国的7家实验室进行3次蛋白质的平行样检测,蛋白质检测方法为国家标准方法。结果所有蛋白质检测结果筛查合格率为89.7%。蛋白质检测相对标准偏差为：实验室内0.36%-2.79%,实验室间1.79%-10.10%,室内和室间总和为2.33%-10.47%,14种食品平均4.52%。蛋白质检测的相对扩展不确定度由4.46%-19.60%,平均8.65%。结论我国实验室对蛋白质的检测符合国家标准方法的要求,实验室蛋白质分析变异和不确定度均小于检测值的20%。
Objective To observe the variety of protein analysis of prepackaged foods from different labs of China. Methods Fourteen foods from seven kinds （cereal products, bean products, meat products, dairy products, beverages, nuts, and snacks）were selected as food samples, according to national prepackaged foods consumption survey results in 2000. These samples protein were parallelly analyzed for three times by national standard methods in seven labs from all the country. Results The 89.7% of results were up to grade by Grubbs, Cochran and Dixon screening methods. The accuracy of protein analysis was expressed by variety coefficiency （ CV）. The CV of inner labs and inter labs were from 0.36% to 2.79% and from 1.79% to 10.10%, the total and average CV level of inner labs and inter labs were from 2.33% to 10.47% and 4.52%. The relative expanded uncertainty of protein detection was from 4.46% to 19.60% （ average 8.65 % ）. Conclusion Protein analysis level in all labs from the country could be fit for national standards, the variety and uncertainty of protein analysis in all labs from the country could be no more than 20 %.
出处
《卫生研究》
CAS
CSCD
北大核心
2007年第4期468-471,共4页
Journal of Hygiene Research
基金
达能营养中心膳食营养研究与宣教基金(No.DIC2004-01)
中国营养学会"营养科研基金"2006年项目
关键词
包装食品
蛋白质分析
变异和不确定度
prepackaged food, protein analysis, variety and uncertainy
