摘要
用高效液相色谱法对氟哌啶醇、氯氮平、阿普唑仑、丙咪嗪、阿米替林、氯丙嗪、泰尔登、氟奋乃静、地西泮等９种常用精神病治疗药物过量中毒进行定量分析。标本经一步液－液提取后，在Ｃ８柱上进行分离，流动相为甲醇－乙腈－水，紫外检测波长为２５４ｎｍ，９种药物在１７ｍｉｎ内即可实现良好分离，线性范围为０．５～１６μｇ／ｍｌ，绝对回收率为７６．８％～９３．３％，最低检测浓度为０．１～０．４μｇ／ｍｌ，日内和日间精密度小于７．５％。本法具有简便灵敏、快速准确的优点，适合于临床检测。
HPLC method for determination of nine psychotherapeutic drugs.haloperidol,clozapine,alprazolam,amitriptyline,imipramine,chlorpromazine,diazepam,chlorprothixene,fluphenazine in plasma was developed.The sample was extracted by one step liquid liquid extraction followed by separation on C 8 column.The mobile phase was a mixture of methanol∶acetonitrile∶water ( 16.0 ∶ 36.5 ∶ 47.5 ).The UV detector wavelength was set at 254nm.Nine drugs were effectively separated in 17 minutes.The standard curves of all nine drugs were linear over the range of 0.5 ￣16μg/ml.The absolute recoveries were ranged from 76.8% to 93.3% .The detection limits were in the range of 0.1 ￣ 0.4 μg/ml.The within day and between day RSD were less than 7.5% .This method is simple,sensitive,rapid and accurate.It is satisfactory for the determination of drug overdose.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1997年第4期159-161,共3页
Chinese Journal of Hospital Pharmacy
关键词
抗精神病药
药物中毒
血药浓度
高效液相色谱
antipsychotics,antidepressants,antianxiety drugs,drug poisoning,HPLC
