摘要
为探明大骨节病病区黄腐酸（Fulvicacid，FA）对软骨内成骨过程中矿化的影响，采用4-甲基伞形酮磷酸酯（4－MethylumbelliferylPhosphate，4－MUP）为碱性磷酸酶（AlkalinePhosphatase，ALPase）作用底物，并结合Gomori染色法，研究了FA对体外培养的肥大软骨细胞及基质在矿化过程中ALPase活性影响。发现与视黄酸（Retinoicacid，RA）相似，FA可促进ALPase活性增强（P＜O．05），但过高浓度的FA（＞100mg／L）使ALPase活性下降。
In order to explore the effects of fulvic acid (FA) from Kashin-Beck Disease (KBD) area on mincralization of bone formation occurs by a process of endochondral ossification, 4-Methylumbelliferyl Phosphate (4-MUP) was taken as the substrate of the alkaline phosphatase(ALPase) to determine the activity of the ALPase. At the same time the activity of ALPase was identified by Gomori staining. The study showed the effects of FA from KBD area on the activity of ALPase during mineralization in vitro of cultured hypertrophic chondrocytes and that the matrix is similar to that of retinoic acid. FA from KBD area can increase the ALPase activity (P<O. 05). When the FA concentration is too high (> 100mg/L),the ALPase activity declines.
出处
《地方病通报》
1997年第1期4-7,共4页
Endemic Diseases Bulletin
基金
国家"八五"科技攻关项目!85-917-01004
关键词
黄腐酸
碱性磷酸酶
大骨节病
软骨细胞
Fulvic acid: Alkaline phosphatase
Kashin-Beck Disease
Chondrocytes
