摘要
应用125Ⅰ标记放免法对比测定病区补硒（200μgSe／d，亚硒酸钠，12周）和未补硒居民血小板TXA2生成及血浆TXA2和前列环素（PGI2），用2，3一二氨基萘荧光法和谷胱甘肽还原酶偶联法测定硒含量和GSH－Px活性。结果表明：（1）补硒组红细胞硒和GSH－Px活性显著高于未补硒组（P＜0．01）；（2）补硒组和未补硒组血浆PGI2水平之间无显著差异（P＞0．05）；（3）补硒组血浆TXA2含量、TXA2／PGI2。比值和血小板TXA2生成均显著低于未补硒组（P＜0．01）；（4）血浆TXA2含量和血小板TXA2生成呈显著正相关（r=0．825，P＜0．01）；红细胞硒含量和GSH－Px活性分别与血浆TXA2含量或血小板TXA2生成呈显著负相关（r=-0．696，－O．780，－0．676，－0．796，P＜0．01）。研究说明，给病区低硒居民补硒可减少其血小板TXA2生成，使血浆TXA2水平也下降，两者的降低与补硒后硒状态的增高有密切关系。
To investigate the effects of selenium supplementation on plasma and platelet thromboxane A (TXA2 ) of residents in a Keshan disease area,plasma TXA2,prostacyclin(PGI2) and platelet TXA2 formation were measured of selenium-supplemented(200μg Se/d,as sodium selenite for 12 weeks) and control residents in the endemic area by using125 I radioimmunoassay. Selenium contents were determined by 2, 3-diaminonaphthalene fluorospectrometry, and glutathione peroxidase (GSH-Px ) activities by GSH reductasecoupled method. The results showed that: (1) Erythrocyte selenium contents and GSH-Px activities were significantly higher in the supplemented group than in the control; (2) No significant difference between plasma PGI2 contents of the two groups was observed; (3) Plasma TXA2, TXA2/PGI, ratio and platelet TXA2 formation were remarkably lower in the supplemented group than in the control; (4) A significant positive correlation between plasma TXA2 and platelet TXA2 formation, and significant negative correlations between erythrocyte selenium contents or GSH-Px activities and plasma TXA2 contents or platelet TXA2 formation were obtained. These data indicate that selenium supplementation results in decreases of plasma TXA2 levels and platelet TXA2 formation in resident with low-selenium status, and both the decreases are closely related to the elevation of selenium status after selenium supplementation.
出处
《地方病通报》
1997年第1期1-3,共3页
Endemic Diseases Bulletin
基金
国家自然科学基金!39270611
关键词
克山病
补硒
血小板
血栓素
前列环素
硒
Keshan disease
Platelets
Thromboxane
Prostacyclin
Selenium
