摘要
采用免疫组织化学和胞浆斑点杂交法，研究了γ射线照射后SMMC－LTNM肝癌细胞DNA聚合酶β基因转录和表达的变化。结果显示，肝癌细胞在体照射2Gy、4Gy后24～48h，细胞内该酶基因表达增强，酶含量增加，照射后72h基本恢复至照射前状况；该酶基因转录也出现增强，其mRNA含量有一过性升高，照射后36～48h达峰值，约为照射前水平的1．76倍。这些结果提示，γ射线照射后肝癌细胞内DNA聚合酶β的作用得到加强，可能对肿瘤的放疗产生不利影响。
Upon the previous studies about the effect of radiation on the DNA polymerase β (pol β) activity and the roles of the enzyme on DNA repair synthesis, we reported the effects of γ-irradiation on gene transcription and its expression of DNA pol β. After 2 or 4 Gy total body irradiation, some biological properties of the enzyme in SMMC-LTNM hepatoma cells borne in nude mice changed. The content of DNA pol β mRNA in hepatoma cells increased significantly, reached the maximum (about 1.76-fold of control) at 36 hours, then decreased closely to control level 3 days later. The gene expression tested by immuno-gold-silver staining assay exhibited some alterations which showed that the enzyme gene expression become more active at 48 hours after irradiation. These results indicated that γ-irradiation would promote DNA pol β transcription and enhanced its DNA repair function.
出处
《辐射研究与辐射工艺学报》
EI
CAS
CSCD
北大核心
1997年第1期44-47,共4页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金
关键词
DNA聚合酶Β
基因转录
肝癌
DNA polymerase β, Gene transcription, Gene expression, Gammarays, SMMC-LTNM hepatoma
