摘要
目的观察^(125)Ⅰ粒子近距离照射对H22肝癌细胞凋亡的影响及其机制。方法用原位末端脱氧核苷酸转移酶标记法(TUNEL)检测^(125)Ⅰ粒子近距离照射对H22细胞凋亡的影响;免疫组化Elivion ^(TM)plus法检测Survivin蛋白的表达。60只小鼠分为A组:植入放射性粒子;B组:植入化疗药DDP;C组:植入放射性粒子和化疗药DDP;D组:正常对照组。结果^(125)Ⅰ粒子近距离照射和/或化疗后对H22肝癌肿瘤体积抑制率A、B、C组分别为43.8%、40.7%和58.3%;凋亡指数(AI)分别为(25.15±10.36)、(33.42±12.25)和(42.34±13.95),与对照组D组(20.45±14.54)比较,C组其凋亡指数(AI)差异有统计学意义(P<0.05);Survivin蛋白的表达率分别为50.0%、55.6%和36.4%,与D组100.0%比较,C组表达率差异有统计学意义(P<0.05)。结论^(125)Ⅰ粒子近距离照射H22肝癌可有效诱导肝癌细胞凋亡,抑制肝癌细胞增殖;联合化疗药,其凋亡作用更强;Sur- vivin可能参与了其中的调节作用。
Objective To investigate the effects and mechanisms of H22 hepatoma cell apoptosis induced by radioactive seeds ^125I. Methods The H22 hepatoma cell apoptosis induced by radioactive seeds ^125I brachytherapy was measured by Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling （TUNEL） method,and immunohistochemical stain （ ElivionTM plus） was used for detecting the protein expression of Survivin. Sixty mice were randomly divided into 4 groups （n = 15 in each group） ： group A （interstitial radioactive seed ^125I） ,group B （DDP） ,group C （interstitial radioactive seed ^125I plus DDP） ,group D （control group）. Results The tumor volume inhibition rate in groups A,B and C was 43.8% ,40.7% and 58.3% respectively. Apoptosis index （AI） in groups A,B and C was （25.15 ± 10.36）, （33.42 ± 12.25） and （42.34 ± 13.95） respectively. The AI in group C was significantly higher than in group D （20.45 ± 14.54） （ P 〈 0.05 ）. The protein expression rate of Survivin in groups A, B and C was 50.0% ,55.6% and 36.4% respectively. The expression rate of group D （ 100% ） was significantly higher than in group C （P 〉 0.05）. Conclusion The seed ^125I brachytherapy induces hepatoma cell apoptosis and inhibits hepatoma cell proliferation. Combined with chemotherapy, ^125I induces stronger apoptosis. Survivin may play a role in the regulation of apoptosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第7期797-799,共3页
Chinese Journal of Experimental Surgery
基金
广西科技厅科学基金(桂科基0575064)
