SLE患者外周血淋巴细胞凋亡及Caspase表达研究被引量：3

Apoptosis and Expression of Caspase in Peripheral-blood Lymphocyte(PBLC) of Systemic Lupus Erythematosus(SLE) Patients

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摘要目的研究SLE患者外周血淋巴细胞凋亡及细胞凋亡caspase途径在SLE病人中作用机制。方法选取SLE患者25例,其中活动期11例,缓解期14例,另设对照组18例;采用体外细胞培养法检测外周血淋巴细胞(PBLC)细胞凋亡率、Westernblot法检测PBLCCaspase蛋白表达。结果SLE患者外周血淋巴细胞凋亡异常,培养前低于对照组,培养24、48h后则明显高于对照组,其差异均有统计学意义;其Caspase-2、3蛋白表达水平由低到高依次是:活动组<缓解组<正常组。结论SLE患者的外周血淋巴细胞Caspase2、3蛋白水平低于正常对照组是自身外周血淋巴细胞凋亡障碍导致的结果。 Objective To study the apoptosis of PBLC in SLE patients and the roles of caspase in the pathogeneses of SLE. Methods PBLC apoptosis was evaluated by vitro cell culture in 25 patients with SLE（ 11 active stage,14 catabasis） , and 18 controls. The expression of caspase of PBLC was also detected by Western blot. Results In SLE, compared with controls, the apoptosis of PBLC were reduced before cell culture while those were increased after culture 24 and 48hrs. Expressions of caspase - 2,3 in active stage patients were lower than those of catabasis group and controls. Conclusions Abnormal PBLC apoptosis results in decreasing Caspase2,3 in SLE patients.

作者徐爱芳许志良

机构地区杭州市第六人民医院浙江中医药大学附属一院

出处《医学研究杂志》 2007年第7期87-89,共3页 Journal of Medical Research

基金浙江省中医药科技计划资助项目(2004C035)

关键词系统性红斑狼疮细胞凋亡外周血淋巴细胞半胱氨酸天门冬氨酸蛋白酶 Systemic lupus erythematosus（SLE） Apoptosis Peripheral - blood lymphocyte（PBLC） Caspase

分类号 R735 [医药卫生—肿瘤]

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参考文献8

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