摘要
目的：探讨血清肝细胞生长因子（ｓＨＧＦ）和转化生长因子－β＿１（ＴＧＦ－β１）水平在病毒性肝炎患者中的临床意义。方法：选１３９例病毒性肝炎患者，以双抗体夹心ＥＬＩＳＡ检测ｓＨＧＦ，以改良ＭＶ１ＬＵ细胞生长抑制ＭＴＴ法检测ＴＧＦ－β＿１活性，同时检测其肝功能、肝纤维化、甲胎蛋白等指标。结果：各型病毒性肝炎患者ｓＨＧＦ水平及ＴＧＦ－β＿１活性均明显高于正常人水平（Ｐ值均＜０．０１）。ＴＧＦ－β１活性增高以肝硬化和重症肝炎最为显著（分别为９．４４±２．１７ｎｇ／ｍｌ和８．４２±２．５４ｎｇ／ｍｌ）。ｓＨＧＦ水平与ＴＧＦ－β＿１活性相关（Ｐ值＜０．０１）。结论：ｓＨＧＦ水平能反映患者肝细胞损伤及肝功能障碍程度，并与肝纤维化程度也可能有关。
Aim: To investigate the clinical significance of Serum Hepatocyte Growth Factor/Scatter factor (sHGF/SF ) and transforming growth factor-β1(TGF-β1) in patients with viral hepatitis. Method: Serum HGF were detected by a “sandwich” enzyme-linked immunosorbent assay in 139 patients with viral hepa- titis and 20 normal subjects, TGF-β1 activity were determined by the modified growth inhibition of MV1LU cells with MTT. some liver function, fibrogeneis markers and AFP concentrations were also meas- ured at the same time. Results: The sHGF levels and TGF-β1 activity in various types of viral hepatitis were obviously higher than those in normal controlls(all P<0.01). Elevation of TGF-β1 activity in LC and FH was the most remarkable (9.44± 2.17ng/ml and 8.42±2.54ng/ml). The sHGF levels showed a positive relationship with TGF-β1. Conclusion: The sHGF levels can reflect the degrees of liver dysfunction, hepatocyte injury and hepatic fibrogeneis. The detection of sHGF levels in patients with hepatitis is impor- tant for predicting of patients condition and prognosis.
出处
《中华肝脏病杂志》
CAS
CSCD
1997年第2期101-102,共2页
Chinese Journal of Hepatology
关键词
病毒性肝炎
肝细胞生长因子
转化生长因子-β_1
Virus hepatitis Serum human heptocyte growth factor Transforming growth factor-β1
