摘要
目的：对３８例人肝细胞癌（ＨＣＣ）及癌旁组织、５例正常肝组织作层粘连蛋白（ＬＮ）及其受体（６７ＬＲ）和Ⅳ型胶原（ＣｏｌⅣ）定位和定量研究。方法：应用免疫组织化学及图像分析。结果：高分化组ＬＮ和ＣｏｌⅣ围绕梁索或腺样结构呈连续线型分布，中、低分化组呈段、点状，ＨＣＣ浸润前缘两者残缺。６７ＬＲ主要于癌细胞膜表面和／或胞浆内表达，其表达水平明显高于癌旁及正常肝组织，尤以低分化组为突出。１８／３８例ＨＣＣ伴肝内浸润和／或转移者，其原发灶内６７ＬＲ表达明显高于无肝内浸润和转移组（Ｐ值＜０．０１）。静脉内癌栓瘤细胞更多表达胞膜型６７ＬＲ。结论：基膜蛋白及ＬＮ受体表达变化与ＨＣＣ浸润、转移和细胞分化程度相关。
Aim: To study the expression of laminin, its 67 kD receptor (67LR) and type N collagen (Col Ⅳ ) in human hepatocellular carcinoma (HCC). Methods: LN 67LR and Col Ⅳ were immunohistochemically localized and quantitated by image anelysis in tumor and peripheral liver tissues from 38 cases of HCC. Results: LN and Col Ⅳ were presented in continuous linear pattern around the trabecular or acinar structures in well-differentiated HCC, while only fragmentary or interrupted Spotty pattern could be detected in mod- erately-and poorly-differentiated HCC . 67LR were localized in either cytoplasm or menbranous surfaces of HCC cells. ln contrast with the paracancerous liver tissues, higher expression of 67LR was found in HCC tissues, especially in poorly-differentiated HCC. The incresed expression of 67LR was observed in 18 cases of HCC with invasion and/or intrahepatic metastasis compared to those without invasion or metastasis (P<0.01 ). and the cytomembrane positive 67LR tumor cells was observed predominantly. in the intravenous tumor emboli Conclusion: The changes of expression of LN and its receptor and Col Ⅳ might be corrdated with invasion, metastasis and cell differentiation of HCC .
出处
《中华肝脏病杂志》
CAS
CSCD
1997年第2期91-93,共3页
Chinese Journal of Hepatology
关键词
肝细胞癌
层粘连蛋白
Ⅳ型胶原
肝肿瘤
Hepatocellular carcinoma Laminin Collagen Ⅳ Laminin reeeptor
