摘要
为探讨肝素的非抗凝作用在肾小球疾病中的意义，采用体外细胞培养的方法，观察了人肾小球系膜细胞在不同浓度的肝素刺激后细胞增殖、释放肿瘤坏死因子和乳酸脱氢酶以及产生纤维连结蛋白的变化，并以未加肝素者作对照。结果：经肝素刺激后４８小时和７２小时，细胞摄入四唑蓝后的吸光度均低于对照，当肝素浓度为５０ｍｇ／Ｌ、５００ｍｇ／Ｌ和５０００ｍｇ／Ｌ时，差异有显著意义。在肝素刺激４８小时的系膜细胞上清液中，乳酸脱氢酶水平则均高于对照，并随肝素浓度的增加而升高。细胞上清中肿瘤坏死因子、纤维连结蛋白的含量均无明显改变。提示：肝素能抑制肾小球系膜细胞的体外增殖，增加乳酸脱氢酶的释放，但对肿瘤坏死因子和纤维连结蛋白的产生则无明显作用。
To find out the role of non anticoagulative effects of heparin in treating glomerular diseases, the authous studied changes in the proliferation, lactic dehydrogenase (LDH) releases and tumor necrosis factor (TNF) or fibronectin (FN) synthesis of human mesangial cells cultured in media with or without heparin by means of cell culture technique in vitro. The results showed that A 570 values of the cells in media with heparin at concentrations ranging from 5 mg/L to 5 000 mg/L were lower than controls without heparin either after 48 hour or 72 hour stimulations. The differences were significant at concentrations of 50, 500 and 5 000 mg/L. Levels of LDH in the supernatants of the cells were increased, but TNF or FN were negative. No effects of heparin on TNF or FN synthesis of the cells were found. These findings revealed that heparin inhibits the proliferation of glomerular mesangial cells in vitro and increases LDH release from the cells.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1997年第4期186-189,共4页
Chinese Journal of Pediatrics
