摘要
针对光散射细胞微粒测量中真实形体细胞的取向对测量结果的影响问题,基于R ay leigh-D ebye-G ans理论和双椭球核式(CEM)模型在应用中的差异,建立了CEM的修正模型(M CEM),根据此模型系统讨论了细胞在不同入射角变化情况下其光散射强度分布所产生的变化,得到了不同入射角下有核细胞光散射强度分布与有核细胞相关物理特征量的动态响应关系;此外,对有核细胞光散射强度分布进行拟合,得到了有核细胞光散射强度分布函数。误差分析表明:拟合结果可有效地应用于真实细胞光散射测量中的数值反演计算。
Based on the application difference in Rayleigh-Debye-Gans theory and CEM model, MCEM （modify co-central ellipse model） is established to eliminate the influence of the orientation effect of the shape-cell particles on measurement result in light scattering cell particles. Based on the MCEM theory, the variation of the light-scattering intensity distribution of cells is discussed systematically at the different incidence angles, the dynamic response relationship between the light-scattering intensity distribution and the relevant physical characteristic of the karyocyte at the different incidence angles was obtained. In addition, the scattering intensity distribution function of the karyocyte is acquired by fitting the scattering intensity distribution. The error analysis shows that the fitting result can be used in the numerical inversion calculation effectively for the light-scattering measurement of the real cell.
出处
《应用光学》
CAS
CSCD
2007年第1期115-120,共6页
Journal of Applied Optics
基金
江苏大学创新基金(04cx06)
关键词
光学测量
细胞微粒
光散射
粒度分布
optical measurement
cell particle
light-scattering
granularity distribution
