摘要
目的比较两种分离嗜血杆菌的培养基对呼吸道嗜血杆菌进行鉴定与分型。方法采用进口原料配制的培养基（Ｈ１）和国产原料配制的培养基（Ｈ２）分离２５４份呼吸道标本中的嗜血杆菌，从菌落生长、杂菌抑制和分离率等方面比较两种培养基的分离效果。结果Ｈ１与Ｈ２培养基差异无显著性，副流感嗜血杆菌、流感嗜血杆菌分离率分别为１０．２％、３．５％，儿童、成人嗜血杆菌属，流感嗜血杆菌分离率分别为３６．１％、１３．１％、８．８％、０．５％。９株流感嗜血杆菌中非ｂ型８株，ｂ型１株，均产β－内酰胺酶。结论国产原料配制的培养基可用于嗜血杆菌属的鉴定与分型。
Objectives To compare two media to isolate haemophilus,and understand the classification,distribution and antibiotic susceptibility of haemophilus isolated from the respiratory tract. Methods We used the oxoid medium(H1)and home made medium(H2) to isolate haemophilus in 254 respiratory tract specimens,and compared the two media with colony growing,contamination inhibition and isolation rate of haemophilus.Homemake heartbrain extract was compared with horse blood agar.The isolation of haemophilus,serotyping and antibiotic susceptibility of H.influenzae etc was investigated .Results No significant difference was observed in the three media.The isolation rate of H.parainfluenzae was higher than that of H.influenzae in the respiratory tract specimens.The isolation rates of haemophilus and H. influenzae of children were higher than those of adults.There were more non-b serotypes in H.influenzae;all produced β lactamase .The highest resistance was TMP/SMZ,and attention should be paid to the resistance of ampicillin.Conclusions The isolation effect of H2 is similar as that of H1 and home made heart-brain extract with horse blood agar.It might be well accepted by many clinical microbiological labs in China.
