摘要
目的评价自制不加血液的培养基和传统的羊血巧克力培养基分离嗜血杆菌的效果。方法将流感嗜血杆菌ATCC10211接种于无血液培养基和传统培养基上,计算和比较两种培养基上嗜血杆菌的平均生长指数,并用此两种培养基对225份临床痰标本进行嗜血杆菌的分离,比较两种培养基上嗜血杆菌的分离率。结果无血液培养基与传统巧克力培养基的平均生长指数分别为11.06、8.05,225份痰标本中嗜血杆菌的分离率分别为20%、11.6%,两者有显著性差异(P<0.01)。结论无血液培养基分离嗜血杆菌的方法简便、实用、效果较好,有应用推广价值。
Objective To evaluate the isolating efficacy of the non - blood culture media and the conventional sheep blood chocolate culture media on Haemophilus species. Methods We put haemophilus （ATCC10211） and 22.5 clinical （sputum） specimens on the non- blood and the conventional sheep blood chocolate culture media, then calculated and compared the average growth rate and the isolation rate of haemophilus on the two culture media. Results The average growth rates on the two culture media were 11. 06 and 8.0.5, the isolation rates of haemophilus were 20 % and 11. 6 % respectively （P〈 0.01 ）. Conclusion The non- blood culture media method is simple, practical, effective, and suitable to be applied in isolating haemophilus species.
出处
《实用预防医学》
CAS
2006年第5期1124-1126,共3页
Practical Preventive Medicine
基金
深圳市盐田区科技局(200302302005)
关键词
嗜血杆菌
培养基
研究
Haemophilus
Culture medium
Study
