摘要
目的:探讨Fas/FasL基因蛋白在胃癌发生发展中的作用。方法:应用免疫组化、特定组织蛋白提取及Westem印迹杂交技术,对20例正常胃黏膜,22例癌前病变和35例胃癌组织中Fas/FasL的表达情况加以分析。结果:正常胃黏膜、癌前病变、胃癌组织的Fas阳性率分别为85%、45%、37%;FasL的阳性率分别为20%、27%、46%。Western印迹杂交显示:胃癌细胞产生的Fas蛋白以分子量30 kDa的可溶型Fas(sFas)为主,伴少量的43 kDa膜型Fas(mFas)。结论:Fas在正常胃黏膜和癌前病变、胃癌组织中的表达强度差异有显著性(P<0.05);FasL在胃癌组织和正常胃黏膜、癌前病变中的表达强度差异有显著性(P<0.05)。
Objective： To investigate the possible role of Fas, FasL in human gastric carcinogenesis. Methods： The expression of Fas and FasL protein was examined by immunohistochemistry staining, protein extraction and Westem blot hybridization. Results： The positive rates of Fas in normal epithelial cells, dysplastic cells and carcinoma cells were 85 %, 45 % and 37 %, respectively. The positive rates of FasL in normal epithelial cells, dysplastic cells and carcinoma cells were 20%, 27% and 46%, respectively. The results of Westem-blotring： the MW of Fas is 30 kDa, and the style of Fas is soluble Fas （sFas）. Conclusion： Fas expression is significantly different between normal epithelial cells and dysplastic cells, carcinoma cells （ P 〈 0.05）. FasL expression is significantly different between carcinoma cells and normal epithelial cells, dysplastic cells （ P 〈 0. 05）.
出处
《沈阳医学院学报》
2007年第1期11-12,共2页
Journal of Shenyang Medical College
基金
辽宁省优秀青年科技人才基金项目资助(No.19963010)
