摘要
目的探讨转化生长因子βⅡ型受体(TβRⅡ)在结直肠癌和其同时性肝转移灶中表达的临床病理意义。方法应用SP免疫组织化学方法检测TβRⅡ和CD34在结直肠癌和其同时性肝转移灶的表达,计算机图像分析技术定量测定表达TβRⅡ的阳性细胞率,根据CD34的阳性表达测定肿瘤微血管密度。结果TβRⅡ的表达同结直肠癌的浸润深度、区域淋巴结转移、肝转移、Dukes分期和肿瘤细胞分化程度呈负相关(P<0.05);定性分析结直肠癌原发病灶与其同时性肝转移灶TβRⅡ表达的阳性率差异无统计学意义(P>0.05),而定量分析两组间阳性细胞率,差异有统计学意义(P<0.05);结直肠癌原发灶中TβRⅡ阳性表达组与阴性表达组间的肿瘤微血管密度值比较,差异无统计学意义(P>0.05)。结论TβRⅡ表达的缺失可能在结直肠癌的恶性演进过程中发挥重要作用。
Objective To investigate the clinicopathologic significance of transforming growth factor β receptor Ⅱ （TβRⅡ ） expression in colorectal carcinoma and their synchronous hepmic metastasis. Methods The SP immunohistochemical stain method was used to observe the expression of TβRⅡ and CD34 in the specimens of eoloreetal carcinoma and synchronous hepatic metastasis. The ratio of positive cell expression TβRⅡ was quantitatively mensurated by compute image analysis. The intratumoral mierovessel density （MVD） was measured by CD34 positive expression analysis. Results There were inverse correlation between the degrees of TβRⅡ expression with the depth of invasion, local lymph node involvement, hepatic metastasis, Dukes stage of clinical pathological classification and cell differentiation （P〈0.05）. The positive rate quantity analysis of TβRⅡ between eoloreetal carcinoma and their synchronous hepatic metastasis had no significant different （P〉0.05）. But the ratio of positive cell expression TβRⅡ quality analysis between them had significant different （P〈0.05）. The expression of TβRⅡ in colorectal carcinoma had no correlation with the value of MVD （P〉0.05）. Conclusion The loss of The TβRⅡ expression in eoloreetal carcinoma perhaps plays important effect in the course of malignant development.
出处
《福建医药杂志》
CAS
2007年第3期100-102,共3页
Fujian Medical Journal
关键词
结直肠癌
肝转移癌
转化生长因子βⅡ型受体
免疫组织化学
Colorectal tumor
Hepatic metastasis
Transforming growth factor β receptor Ⅱ
Immunohistochemistry
