摘要
【目的】观察白花蛇舌草对小鼠腹水H22肝癌细胞形态学及腹水CD4+、CD8+T细胞表达的影响。【方法】将SPF级昆明种小鼠随机分成4组,每组10只,分别为模型组、白花蛇舌草组(剂量为25 g/kg)、党参对照组(25 g/kg)、热休克处理组;均采用活体腹水H22肝癌细胞腹腔移植法复制模型;采用涂片、瑞氏—姬姆萨染色及免疫组织化学标记方法观察各组荷瘤小鼠腹水H22肝癌细胞形态学变化及腹水CD4+、CD8+T细胞的表达。【结果】白花蛇舌草组及热休克处理组小鼠活体腹水H22肝癌细胞变形较为严重,胞膜不完整、发泡;部分细胞核见偏移或固缩。白花蛇舌草组腹水CD4+及CD8+T细胞表达均呈增高趋势,党参对照组及热休克处理组分别以CD4+和CD8+T细胞表达为主。【结论】白花蛇舌草能一定程度抑杀小鼠活体腹水H 22肝癌细胞,其机理可能与提高荷瘤小鼠的T细胞免疫功能及具有一定的细胞毒作用有关。
[Objective] To observe the effect of Herba Hedyotis Diffusae （HHD） on the histological features of living murine ascites hepatoma H22 ceils as well as CD4^＋ and CD8^＋ expression in T lymphocytes. [Methods] Special pathogen free （SPF） Kunming mice were randomized into 4 groups： model group, HHD （25 g/kg） group, Radix Codonopsis （RC, 25 g/kg） group, heat-shock group. The murine ascites hepatoma H22 model was established by abdominal transplantation of H22 ceils. The histological features of H22 ceils from living murine ascites were examined through living mice ascites smear and giemsa staining. The CD4^＋ and CD8^＋ expression in ascites T lymphocytes was detected by immunohistochemical method. [ Results] H22 cell deformation such as cell membrane incomplete and vesicant, some nucleus being migration and pycnosis was obvious in HHD and heat-shock groups. The expression of both CD4^＋ and CD^＋ expression in T lymphocytes of HHD groups was increased, but only CD4^＋ expression in RC group and only CD8^＋ expression in heat-shock group was increased. [Conclusion] HHD exerts some inhibitory-killing effect on living murine H22 cells, and its mechanism may be related to the increase of immune function and to the cytotoxic effect.
出处
《广州中医药大学学报》
CAS
2007年第4期313-316,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省"千百十工程"优秀人才基金(编号:Q02026)
