摘要
以酵母AS2.1190为出发菌株,把含有木糖还原酶基因(XYL1)、木糖醇脱氢酶基因(XYL2),以及木酮糖激酶基因(XKS1)的质粒载体pYMIKP-xy127线性化后多拷贝整合进入其基因组,筛选得到基因工程菌株GZ4-127,并对此工程菌株进行葡萄糖、木糖共发酵试验。结果显示GZ4-127比出发菌株的菌体密度提高5%,木糖消耗提高2倍,酒精产率提高12%,说明工程菌已能够有效地利用木糖生产乙醇。
The industrial stain of Saccharomyces cerevisiae AS2.1190 was transformed with the integration vector pYMIKP-xy127 containing the xylose-utilizing genes, XYL1 and XYL2, which encode xylose reductase （XR） and xylitol dehydrogenase （XDH） from Pichia stipitis, and XKS1, which encodes xylulokinase （XK） from S. erevisiae to produce the recombinant strain GZ4-127. Upon transformation, multiple copies of the xylose-utilizing genes were integrated into the genome rDNA locus of S. cerevisiae. Recombinant strain GZ4-127 consumed twice as much xylose and produced 5% more biomass and 12% more ethanol than the parent strain over 78 h. The results demonstrate that recombinant stain can produce ethanol by xylose fermentation.
出处
《甘蔗糖业》
2007年第3期26-29,共4页
Sugarcane and Canesugar
基金
广东省甘蔗改良与生物炼制重点实验室启动项目"甘蔗渣预处理技术及酒精发酵工艺研究"。
关键词
酿酒酵母
木糖
燃料乙醇
S. Cerevisiae
Xylose
Fuel ethanol
