摘要
建立了室温离子液体四氟硼酸1-丁基-3-甲基咪唑（[Bmim]BF4）和NaH2PO4组成的双水相萃取体系并用于对芦丁的萃取分离研究。考察了离子液体用量、芦丁的浓度、盐的加入量、溶液酸度和加入其它物质对芦丁在两相中分配的影响。结果表明,离子液在1.0-2.5 mL,磷酸二氢钠加入量在1.0-2.0 g,加入卢丁溶液0.5-2.5 mL,酸度在pH值为2-7范围,卢丁在离子液体双水相体系中有较高的萃取率（E%〉90）。除阳离子表面活性剂外,其余大部分物质不影响相比和卢丁的测定。离子液相中卢丁的最大吸收波长为358 nm,与乙醇水溶液中比较,最大吸收波长发生紫移,表明离子液与卢丁发生了作用。利用离子液体双水相体系,测定了银杏叶中卢丁的含量。
A new method of extracting and separating rutin in an aqueous two-phase system （ATPS） formed from water - soluble ionic liquid [ Bmim ]BF4 （1-butyl-3-methylimidazoliumtetrafluoroborate） and phase-forming salt Nail2PO4 at room temperature was studied. The effect of concentrations of ionic liquid, salt added, rutin and solution acitity on the partition of rutin were investigated systematically. In a pH 2 -7 ionic liquid- ATPS system of a total volume of 5 mL formed from 0. 5 - 2.5 mL of 80% alcohol solution of rutin, 1.0 N 2.5 mL [ Bmim] BF4 and 1.0 -2.0 g of NaH2PO4, the ionic liquid phase had the highest extraction efficiency of 90%. Moreover, a lot of foreign substances did not interfere with the phase ratio and the determination of rutin except for cationic surfactants. Compared to that for a ethanol-water solution, the maximum absorption wavelength of the rutin in ionic liquid shifts to lower wavelength, indicating the interaction of rutin and ionic liquid. This method can be applied to the determination of rutin in ginkgo leaves.
出处
《应用化学》
CAS
CSCD
北大核心
2007年第7期838-840,共3页
Chinese Journal of Applied Chemistry
基金
安徽省教育厅自然科学研究项目(2004kj319)
关键词
离子液体
双水相
芦丁
分配
性能
liquid, aqueous two-phase, rutin, partition, character
