摘要
于小鼠腹腔接种ＲＨ株弓形虫速殖子后２ｈ开始，采用乙胺嘧啶和复方磺胺甲口恶唑联合疗法持续治疗５２天，然后用ＰＣＲ与小鼠接种法检测小鼠脑和脾脏中的弓形虫。结果显示：ＰＣＲ与小鼠接种法的检出率分别为６３．６４％和１８．１８％（Ｐ＜０．０５）。提示ＰＣＲ在抗弓形虫药物筛选与疗效考核的体内试验中具有较大应用价值；使用上述两药联合治疗弓形虫病应有足够长的疗程。
Treatment with pyrimethamine and sulfamethoxazolum combination administered via intragastric route, was started 2 hours after each mouse received an intraperitoneal injection of a lethal inoculum of 5×10 3 tachyzoites of RH strain toxoplasma gondii and the therapy was lasted for 52 days. Combined therapy of the two drugs resulted in 83.3% survial rate in mice. PCR and the conventional mouse inoculation (MI) were compared for their validity as an curative effect assessing method. The positive rates of PCR and MI were 63.64% and 18.18%, respectively. The results suggest that PCR can be used in studies involving drug screening and curative effect assessment of anti Toxoplasma chemotherapy in vivo. In addition, the results indicated that longer course of treatment against toxoplasmosis is required in clinical practice.
出处
《湖南医科大学学报》
CSCD
1997年第1期5-7,共3页
Bulletin of Hunan Medical University
关键词
弓形体病
药物疗法
聚合酶链反应
评价
toxoplasma
chemotherapy
curative evaluation studies
polymerase chain reaction
disease models, animal
mice
