摘要
目的：测定米雅利桑爱儿Ａ颗粒中维生素Ｂ６、泛酸钙、维生素Ｂ２的含量。方法：采用高效液相色谱法，用ＷａｔｅｒｓＣＬＣＯＤＳ柱，以乙腈０．２５ｍｏｌ·Ｌ－１磷酸二氢钾（ｐＨ３．２±０．４）为流动相梯度洗脱，对乙酰氨基酚为内标，泛酸钙检测波长为２１０ｎｍ，维生素Ｂ６、内标和维生素Ｂ２的检测波长为２８０ｎｍ。结果：维生素Ｂ６、泛酸钙、维生素Ｂ２分别在２０～１３０μｇ·ｍｌ－１（ｒ＝０．９９９３，ｎ＝６），２０～１３０μｇ·ｍｌ－１（ｒ＝０．９９８６，ｎ＝６），５～３０μｇ·ｍｌ－１（ｒ＝０．９９９３，ｎ＝６）浓度范围内呈线性关系。维生素Ｂ６、泛酸钙、维生素Ｂ２平均回收率分别为９９．７％，ＲＳＤ＝０．８６％；１００．０％，ＲＳＤ＝０．９５％；９８．２％，ＲＳＤ＝１．０２％。结论：方法简便、快速、准确、重现性好。
OBJECTIVE:To make an assay of vitamin B 6,calcium pantothenate and vitamin B 2 in Miyarisan Aiji A fine granules.METHOD:A HPLC method was chromatographed on Waters CLC ODS column with a mobile phase of acetonitrile 0.25 mol·L -1 potassium dihydrogen phosphose(pH 3.2±0.4)(varying from 8∶92 to 25∶75 in 25 minutes),acetaminophen as internal substance.UV detections were performod at 210 nm for calcium pantothenate and 280 nm for vitamin B 6,acetaminophen and vitamin B 2.RESULTS:The standard curves for vitamin B 6,calcium pantothenate and vitamin B 2 were linear in the range of 20～130 μg·ml -1 (r=0.999 3,n=6),20～130 μg·ml -1 (r=0.998 6,n=6),5～30 μg·ml -1 (r=0.999 3,n=6) respectively.The average recoveries of vitamin B 6,calcium pantothenate and vitamin B 2 at different concentration were 99.7%,,100.0% and 98.2% respectively,their RSD was 0.86%,0.95%,1.02%(n=5).CONCLUSION:The method is convenient,rapid,accurate and precise.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1997年第2期106-108,共3页
Chinese Pharmaceutical Journal
关键词
高效液相色谱
米雅利桑爱儿A
颗粒
维生素B6
HPLC,Miyarisan Aiji A fine granules,vitamin B 6,calcium pantothenate,vitamin B 2,assay
