摘要
采用ＭＴＴ法测定人胎儿垂体培养上清（ＦＰＳ）刺激胎脾淋巴细胞增殖反应。实验中观察到：（１）ＦＰＳ能刺激胎脾淋巴细胞增殖，其刺激能力随胎龄增大而增强；（２）胎儿垂体能在体外培养１ｍｏ内持续分泌对淋巴细胞增殖的有效刺激成分；（３）ＦＰＳ能延长胎脾淋巴细胞体外培养存活时间；（４）ＰＨＡ对ＦＰＳ刺激胎脾淋巴细胞增殖无影响。实验结果显示ＦＰＳ对人胎脾淋巴细胞在体外有刺激增殖作用。
The proliferation activity of fetal spleen lymphocytes stimulated with the culture supernatant of human fetal pituitary(FPS) was measured by MTT.The following results were observed:1.FPS had an effect on stimulating the proliferation of fetal spleen lymphocyte,the effect increased as the age of fetus increased;2.Some effective component to stimulate the proliferation of fetal spleen lymphocyte was found in the supernatant of fetal pituitary cultured in vitro for one month;3.The survival time of fetal spleen lymphocytes(cultured in vitro) was prolonged by FPS;4.No effect of PHA on stimulating the proliferation of fetal spleen lymphocytes was found.The results of this study indicated that FPS had an effect on stimulating the proliferation of fetal spleen lymphocytes in vitro.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1997年第1期20-22,共3页
Immunological Journal
关键词
人胎垂体
脾淋巴细胞增殖
胎脾
Human fetal pituitary,Culture supernatant,Spleen lymphocyte proliferation
