摘要
目的：研究视网膜母细胞瘤获得性多药耐药现象，探讨其产生机制。方法：利用长春新碱（ｖｉｎｃｒｉｓｔｉｎｅ，ＶＣＲ）对人视网膜母细胞瘤细胞系ＨＸＯ－ＲＢ４４细胞在体外进行浓度递增法逐步诱导实验，建立一株耐长春新碱视网膜母细胞瘤细胞亚株ＨＸＯ－ＲＢ／ＶＣＲ。并对其细胞生长，药物含量，药物敏感性与放射性敏感性进行检测。结果：该耐药细胞株ＨＸＯ－ＲＢ／ＶＣＲ细胞对长春酰胺、丝裂霉素Ｃ、阿霉素、足叶乙甙、顺铂、卡铂等有交叉耐受，对卡氮芥、氟尿嘧啶无明显抗药性，而对氨甲喋呤的敏感性反而增加。对６０Ｃｏγ射线有轻度耐受性。细胞内药物含量测定显示：耐药细胞内长春新碱浓度明显低于敏感细胞，钙通道阻断剂异博定可使细胞内ＶＣＲ浓度增加，部分逆转其耐药性。结论：ＶＣＲ可诱导视网膜母细胞瘤产生多药耐药和轻度放射耐受。
PURPOSE:Studying the multidrug resistance(MDR) phenotype occurring in retinoblastoma and its mechanism.METHODS:Using the procedure of stepwise increase in drug concentrations to obtain a retinoblastoma subline which resistant to 600ng/ml vincristine (HXO RB/VCR).Characteristics of this drug resistant cell line were investigated by cell counting,drugcontents determinatin,drug sensitivity evaluation and radiation sensitivity test.RESULTS:This cell line was cross resistant to VDS,MMC VP16,ADM,DDP,CBP,but not resistant to BCNU and 5 Fu.It was proved to be collaterally sensitive to MTX,and the response to 60 Co γ ray was modified slightly in HXO RΒ/VCR cell line.Intracellular levels of VCR was much higher in HXO RΒ 44 cells than in the resistant subline.Those cross resistances can be reversed by verapamil partly.CONCLUSIONS:MDR and radiation resistance of retinoblastoma can be induced by exposing to VCR and reversed by verapamil partly.
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
1997年第1期6-9,共4页
Chinese Journal of Ocular Fundus Diseases
关键词
视网膜母细胞瘤
长春新碱
抗药性
细胞株
Retinoblastoma Neoplasms,Experimental Vincristine Drug tolerance
