摘要
本文采用胰酶分散法制备抗原，建立了间接荧光抗体法。用此法对人工感染鸡、人工感染鸡胚及自然发病鸡的抗原进行了测定，结果，人工感染鸡肾脏于７２～１６８ｈ１００％检测到特异性荧光，其余时间部分鸡检出特异性荧光；人工感染后病死鸡肾脏均１００％检测到特异性荧光；人工感染鸡胚于４８～１２０ｈ１００％检测到特异性荧光，其余时间部分检出特异性荧光；１７８例自然发病病例中检出抗原阳性病例１５３例。对抗原检测的结果与冰冻切片制备抗原建立的间接荧光抗体法完全一致。用胰酶分散法制备抗原，对已知阳性血清和待检血清进行检测，检测结果与用冰冻切片法制备抗原检测结果一致。该法将检测程序简化，检测时间明显缩短。
An indirect fluorescent antibody (IFA) technique was developed with the method of trypsinization antigen to detect the antigen in kidneys of chinkens and chick embryos experimentally infected with IBV and in kidneys of dead chickens naturally infected.It was shown that the percentage of bright specific fluorescence (BSF) in kidneys of chickens for 72￣168 hours after artificial infection with IBV was 100%,but at other times percentage was not so high.The percentage of BSF in kidneys of chickens died from artificial infection with IBV was 100%.The percentage of BSF in kidneys of chick embryos for 48￣120 hours after artificial IBV infection was 100%,but at other times was not so high.In 178 cases of chickens naturally infected with IBV, the antigen positive reaction was seen in 153 cases.The results of the trypsinization antigen IFA and the frozen section antigen IFA were identical.The results of detection of the known positive antibody and chickens serum with the trypsinization antigen IFA and the frozen section antigen IFA were identical,too.The trypsinization antigen IFA is simple and more rapid.This method can be used for clinical diagnosis and detection of antibody levels of chickens.
出处
《中国兽医杂志》
CAS
北大核心
1997年第5期3-5,共3页
Chinese Journal of Veterinary Medicine
基金
河南省自然科学基金
关键词
鸡病
肾型
传染性支气管炎
病毒
胰酶分散抗原
Avian infectious bronchitis virus nephropathogenic strain trypsinization antigen technique Immunofluorescence technique
