摘要
目的应用随机扩增多态性DNA（RAPD）技术检测小鼠肝癌H22细胞克隆株的遗传变异。方法用47条引物对小鼠肝癌H22细胞的4个克隆株H22-H8D8,H22-H2G4,H22-H2E10,H22-H2C4基因组DNA进行RAPD分析。结果47条引物均能扩增出明显的条带,条带数多为4~10条,片段大小在200~2000 bp之间。其中4条引物的扩增条带具有多态性,表现为带的有无、移动和强弱差异。结论RAPD技术可以用来检测小鼠肝癌H22细胞克隆株基因组之间的差异。
Objective To detect the genetic stability in four elonal cell strains of murine hepatoma 22 with RAPD analysis. Methods Genomic DNAs from H22-H8D8,H22-H2G4,H22-H2E10,and H22-H2C4 were amplified by RAPD with 47 10bp random primers. Results The most amplified products were monophic, 4 of the 47primers generated polymorphic profile in four clonal cell strains. A muhiband profile were observed for all the primers with the totle number of bands per primer varying from 4 to 10 mostly, and these bands ranged from 200bp to 2kb. The alterations exhibited in the four conal cell lines included the loss of bands, shift of bands and intensity changes of bands. Conclusion Difference in genomic DNAs of four conal cell strains have been detected by RAPD analysis.
出处
《中国比较医学杂志》
CAS
2007年第3期128-130,共3页
Chinese Journal of Comparative Medicine
