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反义及正义Vim重组逆转录病毒表达载体的构建 被引量：1

Construction of Antisense and Sense Vimentin Retrovirus Expression Vector
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摘要 目的构建反义及正义波形蛋白重组逆转录病毒载体,研究波形蛋白在反应性胶质化中的功能与作用。方法应用分子克隆方法克隆、鉴定、包装并感染培养的星形胶质细胞。结果同时获得全长反义及正义Vim逆转录病毒克隆细胞株和较高滴度的病毒上清,反义Vim逆转录病毒抑制培养星形细胞的Vim表达。结论重组的反义及正义Vim逆转录病毒可用于进一步的实验研究。 Objective To construct antisense and sense vimentin retrovirus vector for investigating the effect of vimentin on reactive gliosis. Methods Molecular clone methods were used for tp clone,identifing the antisense and sense vimentin retrovirus and their package.The astrocytes were infected by retrovirus,cultured and determined by SABC method. Results Both the antisense and sense vimentin retrovirus were gained and also high titer of retrovirus superfactant was gotten. The expression of vimentin in the cultured astrocytes was inhibited by antisense vimentin retrovirus compared with sense retrovirus. Conclusion Antisense and sense vimentin retrovirus can be used for the study of reactive gliosis.
作者 林江凯 蔡文琴
机构地区 第三军医大学附属西南医院神经外科 第三军大学基础部组胚教研室
出处 《华南国防医学杂志》 CAS 2005年第1期21-24,F003,共5页 Military Medical Journal of South China
基金 国家自然科学基金资助项目(30471781)
关键词 重组逆转录病毒表达载体 VIM 正义 重组逆转录病毒载体 反应性胶质化 星形胶质细胞 波形蛋白 克隆细胞株 分子克隆 方法应用 星形细胞 病毒抑制 实验研究 高滴度 培养 Vimentin Retrovirus Molecular clone Astrocyte
分类号 R394 [医药卫生—医学遗传学] R651.15 [医药卫生—外科学]
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参考文献2

  • 1[2]Seiter K, Kancherla R, Yang L, et al . Adenovirus and retrovirus mediated interferon alpha gene transfer into CD 34 + cells maintains regeneration capacity and enhances adhesion molecules in K562 cells .J Investig Med, 1999,47(8):414 ～ 424
  • 2林江凯,蔡文琴.波形蛋白在大鼠大脑穿刺损伤后的表达研究[J].第三军医大学学报,2001,23(10):1149-1152. 被引量：5

二级参考文献10

  • 1[1]Lenz G,Manozzo L, Gottardo S, et al. Temporal profiles of the in vitro phosphorylation rate and immunocontent of glial ftbrillary acidic protein (GFAP) after kainic acid-induced lesions in area CA1 of the rat hippocampus: demonstration of a novel phosphoprotein associated with gliosis [J].Brain Res, 1997,764( 1 - 2): 188 - 196.
  • 2[2]Stichel C C, Muller H W. The CNS lesion scar: new vistas on an old regeneration barrier[J]. Cell Tissue Res, 1998,294( 1 ): 1 - 9.
  • 3[3]Kharlamov A, Klarlamov E, Armstrong D M. Age-dependent increase in infarct volume following photochemically induced cerebral infarction: putative role of astroglia [J]. J G erontol A Biol Sci Med Sci, 2000, 55 ( 3 ):B135- 141.
  • 4[4]Horner P J, Power A E, Kempermann G, et al. Proliferation and differentiation of progenitor cells throughout the intact adult rat spinal cord[J]. J Neuurosci.2000,20(6) :2 218- 2 228.
  • 5[5]Miyake T, HattoriT, Fukuda M, et al. Quantitative studies on proliferative changes of reactive astrocytes in mouse cerebral cortex[J]. Brain Res, 1988,451( 1 - 2): 133 - 138.
  • 6[6]Johansson C B, Momma S, Clarke D L, et al. Identification of a neural stem cell in the adult mammalian central nervous system[J]. Cell, 1999,96(1):25-34.
  • 7[7]Mikucki S A, Oblinger M M. Vimentin mRNA expression increases after corticospinal axotomy in the adult hamster[J]. Metalb Brain Dis, 1991,6(1):33-49.
  • 8[8]Ransohoff R M, Tani M. Do chemokines mediate leukocyte recruitment in posttraumatic CNS inflammation[J] ? Trends Neuros, ci, 1998,21 (4): 154 - 159.
  • 9[9]Winter C G, Saotome Y, Levis, on S W, et al. A role for ciliary neurotrophic factor as an inducer of reactive gliosis, the glial response to central nervous system injury[ J ]. Proc Natl Acad Sci USA, 1995,92 (13): 5 865 -5869.
  • 10[10]Acarin L, Gonzalez B, Castellano B. STAT3 and NFkappaB activation precedes glial reactivity in the excitotoxically injured young cortex but not in the corresponding distal thalamic nuclei[J]. J Neuropathol Exp Neurol,2000,59(2): 151 - 163.

共引文献4

同被引文献6

  • 1KIM D H,HEO S D,AHN M J,et al.Activation of embryonic intermediate filaments contributes to glial scar formation after spinal cord injury in rats[J].JVetSci,2003,4(2):109 -112.
  • 2LIN J,CAI W.Effect of vimentin on reactive gliosis:in vitro and in vivo analysis[J].J Neurotrauma,2004,21(11):1671 -1682.
  • 3PEKNY M,LEVEEN P,PEKNA M,et al.Mice lacking glial fibrillary acidic protein display astrocytes devoid of intermediate filaments but develop and reproduce normally[J].EMBO J,1995,14 (8):1590 -1598.
  • 4RIBOTTA M G,MENET V,PRIVAT A.Glial scar and axonal regeneration in the CNS:lessons from GFAP and vimentin transgenic mice[J].ActaNeurochirSuppl,2004,89:87-92.
  • 5GALOU M,COLUCCI-GUYON E,ENSERGUEIX D,et al.Disrupted glial fibrillary acidic protein network in astrocytes from vimentin knockout mice[J].JCellBiol,1996,133(4):853 -863.
  • 6林江凯,蔡文琴.波形蛋白在大鼠大脑穿刺损伤后的表达研究[J].第三军医大学学报,2001,23(10):1149-1152. 被引量：5

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华南国防医学杂志
2005年 第1期

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