摘要
目的:探讨抑癌基因PTEN和癌基因C-myc在结直肠癌发生、发展中的作用及其相关性。方法:采用免疫组织化学链霉菌抗生物素蛋白-过氧化酶法(S-P法),检测60例结直肠癌组织、10例癌旁组织及8例正常结直肠黏膜中PTEN和C-myc蛋白表达。结果:结直肠癌组织中,C-myc蛋白表达阳性率为76.70%(46/60),显著高于正常(0)及邻近结直肠(癌旁)组织(10%),组间比较差异均有显著性(P<0.01);C-myc蛋白过度表达与结直肠癌肝转移呈正相关关系(r=5.540,P<0.05),与TNM分期无相关性(r=0.013,P>0.05);结直肠癌组织中,PTEN蛋白表达阳性率为25.00%(15/60),显著低于正常(100%)及邻近结直肠(癌旁)组织(90%)(P<0.01);PTEN蛋白表达与肿瘤分化程度呈正相关(r=0.869,P<0.05),与TNM分期、淋巴结转移和肝转移均呈负相关(r=-0.791,r=-0.741,r=-0.634,P<0.05);C-myc蛋白表达与PTEN蛋白表达水平呈负相关(r=-0.322,P<0.001)。结论:癌基因C-myc蛋白过度表达不能作为判断结直肠癌肿瘤状态的单独指标;抑癌基因PTEN蛋白的表达缺失在结直肠癌的发生、发展及转移过程中起促进作用,有望成为结直肠癌诊断和预后判断的标记物。
Objective To investigate the correlation between the expressions of tumour suppressor gene PTEN and oncogene C-myc in colorectal carcinoma. Methods The expressions of PTEN and C-myc protein in normal colorectal mucosa （n：8）, adjacent non-cancerous tissues （n= 10） and primary colorectal carcinoma tissues （n= 60） were observed by S-P immunohistochemical assay. Results Of 60 colorectal carcinoma tissues, C-myc protein was detected in 46 cases. The expression rate of C-myc in the primary colorectal carcinoma tissues （76.70%） was significantly higher than that in the normal （0） and adjacent non-cancerous tissues （10%） （P~ 0.01） . Overexpression of C-myc was significantly correlated with hepatic metastasis （r=5. 540, P〈0.05） and was not related with TNM staging （r=0. 013, P〈0.05）. The positive expression rate of PTEN protein in the primary colorectal carcinoma tissues （25.00%, 15/60） was significantly lower than that in the normal （100%） and adjacent non-cancerous tissues （90%） （P 〈 0.01）. PTEN protein expression was positively correlated with pathological differentiational degree （r=0. 869, P〈0.05）, negatively with TNM staging, lymph nodes metastasis and hepatic metastasis （r= - 0. 791, r= 0. 741, r= - 0. 634, P〈0.05）. The level of C-myc protein expression was negatively correlated with PTEN expression （r=- 0. 322, P〈0. 001）. Conclusion C-myc protein overexpression can not be used as a clinicopathological parameter to dignose colorectal cancer. The expression of PTEN is closely related to clinicopathological features in colorectal carcinoma, and could be used as a marker of tumour burden of colorectal carcinoma.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第3期570-573,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技发展计划项目基金资助课题(20030417-02)
