摘要
目的用环磷酰胺造成大鼠的骨丢失,观察丹参骨宝的影响。方法用4.5mg.kg-1.d-1的环磷酰胺灌胃给大鼠,连续15d,造成大鼠骨丢失;用丹参骨宝进行预防给药。实验结束后,取大鼠左侧胫骨上段进行不脱钙包埋切片作骨组织形态计量学测量。同时观察大鼠胸腺、脾脏的重量指数,以及外周血白细胞数量。结果环磷酰胺可使大鼠外周血白细胞数量和脾脏指数明显下降,本实验剂量的丹参骨宝不能有效对抗这种免疫抑制。环磷酰胺通过明显抑制骨形成而导致骨量减少,骨显微结构退化等典型骨质疏松特征的出现。丹参骨宝能通过增加骨的沉积矿化率,促进成骨活性而增加环磷酰胺大鼠的骨量,改善骨微观结构。结论丹参骨宝可有效预防环磷酰胺所引起的大鼠骨丢失,其预防机制与促进骨的矿化沉积率,增加骨形成活性有关。
Objective To study osteoporosis of rats induced by cyclophosphamide and investigate the preventive effects of Danshen Gubao on bone histomorphometry of the rats. Methods Cyclophosphamide at the dose of 4.5 mg/（kg·d） was given to the rats orally for 15 days while Danshen Gubao was given to cyclophosphamide-treated rats orally. At the experimental endpoint, bone histomorphometric analysis of the left shin bone were performed in undecalcified sections. The weights of spleen, thymus and the number of WBC in peripheral blood are also measured. Results The dose of Danshen Gubao in this experiment can not improve the decrease of the index of spleen and the number of WBC, which are induced by cyclophosphamide. Cyclophosphamide causes osteopenia and microstructure degradation in rats, which are the typical features of osteoporosis, by significantly inhibiting bone formation. Danshen Guabao cart increase the number of bone trabecula and improve bone microstructure, through the multiplication of bone mineralization and enhancement of bone formation. Conclusion Danshen Gubao can protect cyclophosphamide-induced trabecular bone loss through stimulating osteoblast bone formation and bone mineralization.
出处
《中国骨质疏松杂志》
CAS
CSCD
2007年第5期308-312,351,共6页
Chinese Journal of Osteoporosis
关键词
丹参骨宝
环磷酰胺
大鼠
骨丢失
骨形态计量学
Danshen Gubao
Cyclophosphamide
Rats
Bone loss
Bone histomorphometry
