摘要
目的建立高效液相色谱测定十取代芦丁硫酸钠含量及有关物质。方法采用XDB-C8色谱柱，流动相：乙腈-25 mmol.L^-1TBAB缓冲液（KH2PO410 mmol.L^-1，三乙胺调节pH7.5）=48∶52，流速：1.0 mL.min^-1，检测波长：λ=254 nm;柱温：25℃。结果在2.0-200.0μg.mL^-1浓度范围内峰面积与浓度呈现良好的线性关系，回归方程为：A=12158.5C＋43.63，相关系数r=0.999 9。日内精密度和日间精密度均小于3.5%。结论本法专属性良好，符合十取代芦丁硫酸钠的含量测定和有关物质检查的要求。
OBJECTIVE To establish a HPLC method for determination of RDS and relative impurities. METHODS Agilent Cs column was used. The mobile phase consisted of acetonitrile and 25 mmol·L^-1 phosphate buffer solution containing 20 mmol·L^-1 TBAB （ pH 7, 5 ） （48： 52, v/v） with flow rate of 1.0 mL · min^-1, The detection wavelength was set at 254 nm. RESULTS The linear range of cadibration curve was 2.0 -200.0μg· mL^-1 , A = 12158.5C ＋43, 63, r = 0.999 9. The intra-day and inter-day RSD were less than 3.5%, CONCLUSION The proposed method was simple, precise and reproducible; the decomposed product can be separated as well, Therefore, the quality of RDS can be effectively controlled.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2007年第3期227-229,共3页
Chinese Journal of Modern Applied Pharmacy
