摘要
目的研究重组人白细胞介素13(rhIL-13)诱导巨核细胞白血病细胞株Dami细胞STAT6(signal transducers and activators of transcription 6)磷酸化及对Dami细胞GPⅡb表达的影响。方法RT-PCR检测Dami细胞IL-13Rα1 mRNA、IL-4RαmRNA和GPⅡb mRNA;Western blot检测Dami细胞磷酸化STAT6蛋白表达;流式细胞仪检测Dami细胞GPⅡb蛋白表达。结果Dami细胞表达IL-13 Rα1 mRNA;Dami细胞表达IL-4RαmRNA;IL-13作用Dami细胞2 h,STAT6磷酸化;IL-13组Dami细胞GPⅡb表达高于空白对照组(P<0.05)。结论IL-13诱导Dami细胞STAT6磷酸化并上调Dami细胞GPⅡb表达。
Objective To investigate the effect of IL- 13 on the phosphorylation of STAT6 and its correlation with GP Ⅱ b expression in Dami ceil. Methods After Dami ceils were cultured in a serum-free medium for 20 h, the transcriptional levels of IL- 13Rα1, IL-4Rα and GPⅡ b were analyzed using reverse transcription polymerase chain reaction （RT-PCR）. IL-13-induced phosphorylafion of STAT6 was detected by Western blot in Dami cell after the isolation of proteins. The protein levels of GP Ⅱ b was examined by flow cytometry analysis. Results IL- 13Rα1 mRNA and IL-4Ra mRNA were expressed in Dami cells in serum-free medium. The phosphorylafion of STAT6 was induced after 2 h of IL-13 treatment and dephosphorylation of STAT6 was detected after 4 h in Dami cells. GP Ⅱ b expression was up-regulated in the experiment group （IL-13, 100 ng/ml）. Conclusion The phosphorylation of STAT6 is induced by IL-13 in Dami cells. The up-regulation of the GPⅡ b expression is related with IL-13-induced the phosphorylation of STAT6 in Dami cells.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2007年第6期495-498,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金(39860033)
江西省自然科学基金(0140036)
