摘要
自从1987年正式提出组织工程这一概念来以来,培养具有生物学活性组织器官替代物始终是组织工程学的发展方向。目前,虽然一些工程化组织如皮肤、软骨等已被成功构建,并应用于临床,但其他工程化组织如心脏、骨骼肌、肝脏等体积大、功能复杂,移植后难以及时建立血液供应。而及时建立的血管网络对组织器官的存活与功能实现至关重要。为此,国内外一些实验室采用联合细胞培养的方法,观察不同细胞间的相互作用对血管形成的影响。结果表明,联合细胞培养在血管的形成、稳定和成熟方面起着重要作用。
The ultimate goal in the field of tissue engineering has always focused on constructing tissues that may biologically and functionally serve as a substitute for the organs of dysfunction. However, it is still a major challenge in creating thick and complex artificial tissues and cultivating long-lasting and sufficient vascular network in these tissues in vitro, which are capable of incorporating with the host＇s vascular network after transplantation and finally well perform their physiological function. Therefore, vascularization before transplantation plays a significant role in the survival of engineered tissues especially those that highly require sufficient blood supply such as liver, heart, and skeletal muscle. At the same time, new approaches involved in the notion of cell co-culture system aiming for inducing neovascularization before transplantation have been implemented in some laboratories and will be discussed in this review. Furthermore, recent data has showed that such a co-culture approach can effectively facilitate the formation of vessels, regulate the interaction between endothelial cells and mural cells, and promote signal transduction and ultimately the mature of vascular network.
出处
《细胞生物学杂志》
CSCD
2007年第3期317-320,共4页
Chinese Journal of Cell Biology
关键词
联合细胞培养
血管化
组织工程
cell co-culture
vascularization
tissue engineering
