摘要
在水稻Lemont×Dular的重组自交系群体(RILs,F10)中发现了裂颖突变体(split rice glume,srg).利用扫描电子显微镜观察野生型水稻和裂颖水稻的颖花原基分化情况,结果发现,突变体水稻的幼穗在形成内、外稃原基后,有些颖花在形成浆片后并不是直接发育形成雄蕊原基,而是在外稃或内稃原基内侧又分化出浆片原基;有些颖花原基从中间缢裂,缢裂的颖花原基又分别形成类稃片的结构,颖花发育后期,6枚雄蕊发育畸形,并且观察到不同数目的雄蕊.同时以突变体作母本,Dular、Lemont为父本配制杂交组合进行遗传分析,F2表型和χ2测验结果表明,该突变性状受单基因隐性控制.
A floral organ mutant, split rice glume （srg） , was investigated in rice recombinant inbred lines population （ RILs, F10 ）, which was derived from rice variety Lemont x Dular. The morphogenesis of floral organs in both srg mutant and wild-type rice Dular （Oryza sativa L. ） was observed using the scanning electron microscope （SEM）. The results showed that the floral primordia were differentiated under irregular pattern in srg mutant. After regular formation of lemma and palea primordia, the floral primordium of srg formed lodicule primordia inner to both lemma and palea, followed by the formation of stamens and carpel. Alternatively, floral primordia in srg were split to form two or more floral primordia. The number and position of stamens and carpels primordia were malformed. Two F2 populations were made by crossing srg mutant with two rice varieties Lemont and Dular, respectively, and genetic analyses showed that the mutant trait was controlled by a single recessive gene.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2007年第3期225-229,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省自然科学基金资助项目(B0510015)
关键词
水稻
SRG
花器官
扫描电子显微镜
遗传
rice
srg
floral organ
scanning electron microscope
genetics
