摘要
目的研究模拟失重对大鼠血清中细胞因子(TNF-α,IL-6和IL-8)和肺组织中原癌基因C-fos的影响。方法-30°尾部悬吊(TS)大鼠模拟失重生理效应。40只Wistar大鼠随机分为7d对照组(7dCON)、21dCON、TS7d和TS21d4组。血清细胞因子(TNF-α,IL-6和IL-8)及肺组织中原癌基因C-fos的动态表达分别采用酶联免疫吸附试验法(ELISA)和免疫组织化学法检测。结果TS7d和TS21d大鼠血清中TNF-α,IL-6,和IL-8与对照组(CON)比释放明显增高,差异有统计学意义(P<0.01,P<0.05),且TS7d和TS21d大鼠肺组织C-fos的表达水平与对照组(CON)比差异也具有统计学意义(P<0.01,P<0.05)。结论尾部悬吊模拟失重可能引起大鼠肺脏一定程度的损伤,并对血清炎性细胞因子TNF-α,IL-6和IL-8的释放及肺组织C-fos的表达产生影响。
Objective To investigate the effects of simulated weightlessness on TNF-α IL-6 and IL-8 in serum and C-fos on pulmonary tissues in rats. Methods -30° tail-suspension（TS） rats were used as the model to simulate the physiological effects of weightlessness. Fourty Wistar male rats were randomly arranged into 4 groups： control for 7d （7d CON）, 21d CON, TS for 7d （TS7d） and TS21d. The dynamic expresses of C-los in the sera and lung tissues were respectively observed by using ELISA and the immunohisochemical technique. Results The contents of TNF-α, IL-6 and IL-8 in sera of 7d and 21d tail-suspensed rats increased significantly（P〈0.01, P〈0.05）, while the activation level of C-fos in lung tissue of 7d and 21d tail-suspensed rats increased significantly（P〈0.01,P〈0.05）. Conclusion TS resulted in some lung injury of rats, which could have some effects on the pulmonary circulation and function.
出处
《重庆医学》
CAS
CSCD
2007年第12期1152-1154,共3页
Chongqing medicine
基金
军队医药卫生十一五科研基金专项课题(06Z048)
关键词
失重模拟
大鼠
血清
肺脏
细胞因子
weightlessness simulation
rat
serum
lung
cytokines
