摘要
建立了马来酸曲美布汀和昂丹司琼对映体的高效液相色谱拆分方法。使用Chiralpak AD-H手性色谱柱,采用正相洗脱方式,考察了流动相中有机改性剂的种类和浓度、酸碱比(冰乙酸-三乙胺)、柱温及流速等因素对对映体拆分的影响。确定了最佳拆分条件:分离马来酸曲美布汀的流动相为正己烷-异丙醇-冰乙酸-三乙胺(97∶3∶0.1∶0.3),流速为0.6mL/min;分离昂丹司琼的流动相为正己烷-异丙醇-冰乙酸-三乙胺(70∶30∶0.2∶0.4),流速为0.4mL/min,柱温均为20℃。在上述色谱条件下,两种手性药物对映体达到基线分离。
A high performance liquid chromatographic method has been developed for the enantiomeric separation of trimebutine maleate （TBM） and ondansetron （OND） on a Chiralpak AD-H Chiral column under normal phase mode . The influences of the concentration of organic solvent, the proportion of glacial acetic acid and triethyamine, column temperature and flow rate on the enantiomeric separation were investigated. The optimal chromatographic conditions on AD-H column were hexane-isopropanol-glacial acetic acid-triethyamine （97：3： 0. 1：0. 3 ） as mobile phase with a flow rate of 0. 6 mL/min of TBM and hexane-isopropanol-glacial acetic acid- triethyamine （70： 30： 0. 2 ： 0. 4） as mobile phase with a flow rate of 0. 4 mL/min of OND. The column temperature was set at 20℃. Baseline separation was achieved for the resolution of the two chiral drugs under the above chromatographic conditions. The proposed method was simple, reproducible and less-time consuming.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2007年第6期880-882,共3页
Chinese Journal of Analytical Chemistry
关键词
马来酸曲美布汀
昂丹司琼
高效液相色谱
对映体拆分
Trimebutine, ondansetron, high performance liquid chromatography, enantiomeric separation
