摘要
目的研究鹿茸多肽(VAP)-聚羟基乙/聚乳酸(PLGA)复合膜对肌腱愈合和粘连的影响。方法将健康家鸡48只,雌雄各半,随机分成4组,每组12只。A组为空白对照组,B组为PL- GA膜组、C组为低剂量VAP/PLGA膜组,D组为高剂量VAP/PLGA膜组。将家鸡第2、3、4趾Ⅱ区趾浅屈肌腱切除,趾深屈肌腱横断后均用6-0和9-0线以改良Kessler法缝合。A组单纯修复鞘管;B组应用PLGA复合膜包绕吻合口后修复鞘管;C组应用3 mg/g的VAP/PLGA复合膜包绕吻合口后修复鞘管;D组应用15 mg的VAP/PLGA复合膜包绕吻合口后修复鞘管。石膏固定跖趾关节及趾间关节于屈曲位,2、3、4周取材,分别进行大体观察、组织学检查和生物力学测定。结果4组肌腱除A组外,粘连程度无明显差异,A组粘连较严重;C组和D组大体观察、组织学检查和生物力学检查均优于A组和B组,组织学检查D组优于C组,但大体观察和生物学测定无明显差异。结论VAP/PLGA复合膜是一种良好的减轻肌腱术后粘连、促进肌腱愈合的可降解材料。
Objective To explore the effect of VAP/PLGA complex membrane on the healing and preventing adhesion in sutured tendons. Methods Forty-eight chickens were divided into four group randomly, 12 each group： group A served as controls, group B used PLGA, group C used the low dose VAP, group D used the high dose VAP. The second, third and forth toes were chosen as the experimental models and the flexor digitorum superficialis tendons were cut to increase the space of the sheath. The flexor digitorum profundus tendons were sectioned to establish the model. The deep flexor tendon of the second, third and fourth toes were cut and sutured with a single 6-0 nylon stitch by a modified Kessler technique, encapsulated by PL- GA membrane,low dose（3 mg/g） VAP/PLGA membrane and high dose（ 15 mg/g） VAP/PLGA membrane respectively in group B,C,D. The sheath and skin were sutured and fixed. The material was got after second, third, forth week. The specimens were observed under naked eye, microscope and electricmicroscope. Mean- while the stress, strain, ridgity and functional recovery were quantificationally analyzed to guarantee the objectivity of the results. Results The tendon adhesion is no difference in group B and C and D, but group A was more serious than the others ; Group C and D were better than group A and B in observation under naked eye and examination of histological and biological mechanics, and group D was better than group C in histology examination, but they were no difference in observes under naked eye and biological mechanics examination. Conclusion VAP/PLGA complex membrane is a better decomposable substance on accelerating the healing and preventing adhesion in tendon.
出处
《中华显微外科杂志》
CSCD
北大核心
2007年第3期197-199,I0004,共4页
Chinese Journal of Microsurgery
基金
长春市科技计划项目(2004073)
