摘要
目的建立用HPLC测定黄芩素/HP-β-CD包合物经大鼠尾静脉快速注射给药后,黄芩素及其代谢产物黄芩苷血浆浓度的方法。方法采用Agilent 1100 HPLC系统,DiamonsilTMODS色谱柱,以甲醇和磷酸溶液组成的流动相梯度洗脱分离,以对羟基苯甲酸丙酯为内标,流速1.0 mL.min-1,检测波长270 nm,柱温40℃,进样20μL检测。结果在本色谱条件下黄芩素、黄芩苷及其他代谢产物与杂峰分离良好;黄芩素和黄芩苷在0.05～15 mg.L-1内呈线性关系;两者日间和日内精密度均低于11.6%;黄芩素和黄芩苷的最低检测浓度分别为0.03和0.05 mg.L-1。结论该方法可在同一条件下同时检测黄芩素和黄芩苷,且简便、准确、可靠,可用于黄芩素/HP-β-CD包合物静注给药后,其主药黄芩素及其主要代谢产物黄芩苷的血药浓度测定。
OBJECTIVE To develop a RP-HPLC method with 15V detection for the simultaneous determination of baicalein and its major metabolite （baicalin） after intravenous administration of baicalein/HP-β-CD complex, METHODS The Angilent 1100 HPLC system was used in the drug analysis, The samples were separated on a reversed-phase HPLC column （Diamonsil^TM ODS） with the mobile phase consisting of methanol and 0. 2% phosphoric acid solution,using a linear gradient elution program. Propyl paraben was used as internal standard. The flow rate was set at 1 mL·min^-1 and the eluent was detected at 270 nm. The column temperature was 40% and 20 μL sample was injected into the HPLC column, RESULTS Both baicalein and baicalin had good linear relationship over the range of 0.05 - 15 mg·L^-1. The intra-day and inter-day RSD were less than 11.6%. The limits of quantification in plasma of baicalein and baicalin were 0. 03 and 0. 05 mg·L^-1, respectively. CONCLUSION The developed method is simple, accurate and reliable for determining baicalein and baicalin simultaneously in a single chromatographic run.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2007年第12期929-932,共4页
Chinese Pharmaceutical Journal
