摘要
目的探讨选择性头部亚低温对缺氧缺血新生猪脑血流的影响。方法生后7 d龄新生猪16头随机分为三组:缺氧缺血常温组(n=6)、缺氧缺血亚低温组(n=6)和正常亚低温组(n=4)。采用双侧颈总动脉阻断和吸入6%的氧30 min制备新生猪缺氧缺血性脑损伤模型。亚低温组鼻咽部温度分别降到35℃和32℃,常温组维持正常体温(39℃)。采用彩色微球方法测定脑血流。结果正常亚低温组35℃和32℃时全脑血流分别降低到基础值的74%和52%(P<0.05),颞叶、顶叶、脑干和小脑降低较为明显。新生猪缺氧缺血后2 h、4 h和6 h全脑血流较缺氧缺血前降低,差异有显著性意义(P<0.05);缺氧缺血后4 h局部脑血流均显著降低,缺氧缺血后6 h海马、纹状体及丘脑脑血流仍较低(P<0.05)。缺氧缺血新生猪亚低温治疗后,35℃和32℃时全脑血流、局部脑血流与常温组相应的时间点比较差异没有显著性意义。结论新生猪缺氧缺血后脑血流降低。选择性头部亚低温可以显著降低正常新生猪脑血流,但对缺氧缺血新生猪脑血流没有显著影响。
Objective To study the influence of selective head cooling （SHC） on cerebral blood flow （CBF） in newborn piglets with hypoxia-ischemia （HI）. Methods Sixteen newborn piglets, aged 7 d, were randomly assigned to one of the following groups： SHC after HI （n=6）, normal temperature after HI （n=6） and SHC in normal piglets （n=4）. HI was induced by the temporary occlusion of bilateral carotid arteries and mechanical ventilation with low concentration of oxygen （6%） for 30 min. Nasopharyngeal temperature was respectively reduced to 35 ℃ and then 32 ℃ in SHC. 39 ℃ was set as normal temperature. CBF was measured with color microspheres. Results Global CBF （GCBF） decreased to 74% and 52% （P〈0.05） of baseline respectively at 35 ℃ and 32 ℃ during SHC, particularly in the temporal lobe, parietal lobe, brain stem and cerebellum. The GCBF was significantly reduced at 2 h, 4 h and 6 h after HI, compared with that before HI （P〈0.05）; all of regional CBF （RCBF） were significantly reduced at 4 h after HI and at 6 h, RCBF ofhippocampus, striatum and thalamus was still low（P〈0.05）. The hypoxic-ischemic neonatal piglets treated with mild hypothermia, at 35 ℃ and 32 ℃, comparisons of GCBF and RCBF with that of the normothermia group at corresponding time points showed no significant difference. Conclusion The CBF of neonatal piglets would decrease after HI. SHC may significantly reduce CBF of neonatal piglets, but has no significant impact on hypoxic-ischemic neonatal piglets.
出处
《中华神经医学杂志》
CAS
CSCD
2007年第6期550-553,共4页
Chinese Journal of Neuromedicine
基金
复旦大学211工程资助项目
关键词
脑血流
亚低温
缺氧缺血
Cerebral blood flow
Mild hypothermia
Hypoxia-ischcmia
