摘要
利用渗透交联固定化细胞促进生物转化石屹峰金凤燮吴怡莹严复(大连轻工业学院食品工程系大连116001)虞星炬袁权(中国科学院大连化学物理研究所大连116012)固定化技术已在生物工程中得到广泛的实际应用,特别是应用于生物转化以提高酶或细胞的稳定性,...
In order to achieve high activity of enzymatic catalysis,the method of permealizing and crosslinking treatment has been suggested for immobilizing cells with endocelar enzymes.Polyethene polyamine and glutardehyde was used to enhance the activity and stability of E.coli having aspartase entrapped in κ carrageen gel and intact Pseudomonas dacunhae cells having aspartate β decarbonoxylase.The strength and enlargement effects,which favor the performance of catalysis in packed bed reactor and membrane reactor respectively,were also obtained from the experiment results.
出处
《生物工程学报》
CAS
CSCD
北大核心
1997年第1期111-113,共3页
Chinese Journal of Biotechnology
关键词
生物工程
生物转化
渗透交联
固定化细胞
Immobilized cells,\ permealizing,crosslinking,\ polyethene polyamine,\ glutardehyde,\ L aspartic acid,\ L alanine