摘要
利用聚丙烯酰胺垂直平板凝胶电泳法，对普通小麦Ｋ、Ｖ型雄性不育系、保持系在不同发育时期的雄蕊、雌蕊、旗叶的过氧化物酶同工酶进行了系统研究。结果表明，Ｋ、Ｖ型不育系、保持系在不同发育时期的花药具有不同的酶谱。根据酶带数量和酶活性的变化，发现Ｋ、Ｖ型不育系之间在花粉败育时间上有较大差异。认为Ｖ型不育系败育的关键时期是“单核期”，而Ｋ型不育系为“二核期”，与细胞学观察结果相吻合。Ｋ、Ｖ型不育系、保持系之间在不同发育时期的雌蕊。
By means of vertical slab polyacrylamide gel electrophoresis,the isozyme zymograms of peroxidase were analyzed in the stamen,pistil and flag leaf from plants of Ae.kotschyi cytoplasmic male sterile line (K CMS),and of Ae.ventricosa cytoplasamic male sterile line (V CMS) as well as its maintenance line (B) at different developmental stages. The results showed that the isozyme zymograms of anthers of K CMS,V CMS and B were different at various developmental stages. According to the change of the isozyme bands and activity,it was found that the stage of pollen abortion was different between K CMS and V CMS. It was supposed that the key stage of pollen abortion was “single nucleus stage”for V CMS, but “double nucleus stage”for K CMS,This was in accordance with the observation of cytology.The isozyme zymograms of pistil and flag leaf of K CMS,V CMS,B showed no significantly different at different stages.
出处
《华北农学报》
CSCD
北大核心
1997年第2期7-11,共5页
Acta Agriculturae Boreali-Sinica
关键词
小麦
过氧化物酶
雄性不育系
保持系
Common wheat
Peroxidase isozyme
K type male sterile line
V type male sterile line
Maintenance line
