摘要
目的探讨湖南衡阳地区白细胞介素-1B（IL-1B）基因多态性与胃癌的关系以及幽门螺杆菌（Helicobacter pylori,HP）感染后胃癌发生的易感基因型。方法52例胃癌患者癌旁正常胃粘膜组织和55例慢性胃炎患者胃粘膜组织,均经快速尿素酶和PCR检测HP,应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析技术,进行基因型检测,并对C/C、T/T进行测序,比较各基因型在胃癌组和胃炎组中的分布差异。结果IL-1B-31T、IL-1B-511T等位基因和IL-1B-31T/T、IL-1B-511T/T基因型在胃癌组的分布频率高于胃炎组（P〈0.05）,OR值分别为1.97（95%CI=1.15-3.59）、2.52（95%CI=1.45-4.39）和2.71（95%CI=1.10-6.66）、3.33（95%CI=1.14-9.73）。在伴有HP感染的群体中进行比较,IL-1B-31位点各基因型未见明显差异;但IL-1B-511T等位基因和IL-1B-511T/T基因型在胃癌组的分布频率高于胃炎组（P〈0.05）,OR值分别为2.16（95%CI=1.10-4.23）和3.43（95%CI=1.01-11.62）。结论在湖南衡阳地区IL-1B-31T/T、IL-1B-511T/T基因型与胃癌发病风险相关,在HP被感染后IL-1B-511T/T基因型可能为湖南衡阳地区胃癌易感基因型。
Objective To investigate the association of the interleukin 113 （IL-1B） gene polymorphism with gastric cancer in Henyang Chinese population, and to study which type may be the sensitive genotype to gastric cancer in patients with helicobacter pylori （HP）infection. Methods For 52 patients with gastric cancer and 55 patients with chronic gastritis. ,the polymorphism of the IL-1B gene was studied by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis, C/C. T/T genotype were sequenced. The infections of HP were determined by RUT and PCR assay. Results The frequencies of IL-1B-31 T, IL-1B-511 T, IL-1B-31T/T and IL 1B-511T/T genotype were higher in gastric cancer group than in chronic gastritis group （P〈0.05）, with odds ratios（ORs） of 1.97（95%CI = 1.15 -3.59）, 2. 52（95%CI = 1.45-4. 39）, 2. 71（95%CI = 1.10-6. 66） and 3.33（95%CI = 1.14-9. 73）, respectively. In patients with HP infections, the IL-IB-511T and IL IB-511T/T were higher in gastric cancer group than in chronic gastritis group（P〈0.05）, with odds ratios of 2. 16（95%CI = 1.10-4. 23） and 3. 43（95%CI = 1.01 - 11.62）, respectively. Conclusion In the HengYang Chinese population , the IL-IB-31T/T and IL-IB-511T/T genotypes may be associated with the high risk of gastric cancer, and IL-1B-511T/T may be the sensitive genotype of gastric cancer in patients with HP infection.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2007年第6期416-419,共4页
Cancer Research on Prevention and Treatment
基金
湖南省卫生厅基金资助项目(B2003-089)
