摘要
目的研究乙型肝炎(下称乙肝)病毒(HBV)血清标志物、前S2抗原检出情况与HBV-DNA结果之间的关系。方法对472例临床样本采用酶联免疫吸附试验(ELISA)分别进行HBV血清标志物5项、前S2抗原的检测,同时运用荧光定量聚合酶链反应法检测HBV-DNA的含量。结果HBV表面抗原(HBsAg)、HBVe抗原、HBV核心抗原抗体(抗-HBc)阳性组前S2抗原阳性检出率为98.7%和HBV-DNA阳性检出率为84.4%均显著高于HBsAg、抗e抗原抗体、抗-HBc阳性组(P<0.01);159例血清HBV-DNA呈阴性组中前S2抗原检测出67例阳性;130例前S2抗原检测呈阴性组中血清HBV-DNA检出38例阳性。结论前S2抗原、HBV-DNA及HBV血清标志物检测都可对HBV的复制情况进行评估,但并不同步,需联合应用方可对HBV感染、复制情况作出较全面的评估。
Objective To investigate the relationship among HBV markers, pre-S2 antigen and HBV-DNA. Methods 472 eases of clinical species were collected. ELISA was applied to detecting HBV markers and pre-S2 antigen, while FQ-PCR was applied to detecting HBV-DNA content. Results The positive rate of pre-S2 antigen and HBV-DNA was 98.7~ and 84.4% in HBsAg, HBeAg and anti-HBe positive group respectively, higher significantly than that of HBsAg, anti-HBe and anti-HBe positive group （P〈0.01）. In serum HBV-DNA negative group, 67 eases were pre-S2 antigen positive; while in pre-S2 antigen negative group, 38 eases were serum HBV-DNA positive. Conclusion HBV markers, pre-S2 antigen or HBV-DNA can evaluate HBV duplication, but not simultaneous. Combined detection of the three methods should be applied to assessing HBV infection and duplication totally.
出处
《检验医学与临床》
CAS
2007年第6期476-477,共2页
Laboratory Medicine and Clinic
