摘要
利用氨基苄脒修饰的硅胶为亲和吸附剂纯化组织纤溶酶原激活剂（ｔ－ＰＡ），讨论了亲和柱体积对纯化效率的影响．由于氨基苄脒对ｔ－ＰＡ的高度特异性，根据ｔ－ＰＡ的上柱量适当调整柱体积，可使纯化倍数达到１４０以上。
Para aminobenzamidine modified silica was used as an affinity adsorbent in the tissue type plasminogen activator (t PA) purification. The effect of bed volume on the recovery yield and purification factor was studied. If the bed volume was properly lowered depending on the loaded amount of t PA, the purification factor could be increased to over 140. This value was much greater than that obtained by using other affinity adsorbents.
出处
《天津大学学报》
EI
CAS
CSCD
1997年第1期104-107,共4页
Journal of Tianjin University(Science and Technology)
基金
国家教委留学回国人员基金
关键词
纤溶酶原激活剂
氨基苄脒
亲和层析
提纯
tissue type plasminogen activator, para aminobenzamidine, affinity chromatography, purification
